Air Gap Microcell for Scanning Electrochemical Microscopic Imaging of Carbon Dioxide Output. Model Calculation and Gas Phase SECM Measurements for Estimation of Carbon Dioxide Producing Activity of Microbial Sources

• Published in: Electroanalysis (New York, N.Y.) Vol. 23; no. 10; pp. 2320 - 2326
• Main Authors: Kiss, András, Kiss, Laszló, Kovács , Barna, Nagy, Géza
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.10.2011; WILEY‐VCH Verlag
• Subjects: Air gap antimony electrode; Carbon dioxide; Finite element method; Saccharomyces cervisiae; SECM
• ISSN: 1040-0397; 1521-4109
• DOI: 10.1002/elan.201100180

Most of SECM studies have been carried out in liquid or gel phases. A Severinghaus type, CO2 detecting potentiometric tip has been fabricated and used in gas phase SECM studies. The microcell tip is made without gas permeable membrane. Faster response, needed in SECM, was achieved by substituting the membrane with an air gap. The carbon dioxide concentration profile in the gas phase over a disc‐shaped colony of yeast has been detected with the new tip. Estimation of the carbon dioxide output of the fungi colony was attempted by fitting simulated curves to measured ones obtained with one dimensional (line) scans.