Macro- and microelements in the rat liver, kidneys, and brain tissues; sex differences and effect of blood removal by perfusion in vivo

Concentrations of macro- and microelements in animal organs indicate the animal health status and represent reference data for animal experiments. Their levels in blood and tissues could be different between sexes, and could be different with and without blood in tissues. To test these hypotheses, i...

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Bibliographic Details
Published inJournal of trace elements in medicine and biology Vol. 40; pp. 104 - 111
Main Authors Orct, Tatjana, Jurasović, Jasna, Micek, Vedran, Karaica, Dean, Sabolić, Ivan
Format Journal Article
LanguageEnglish
Published Germany Elsevier GmbH 01.03.2017
Subjects
Animals
Brain - metabolism
Female
Gender differences
ICP-MS
Kidney - chemistry
Liver - chemistry
Macronutrients
Male
Micronutrients
Perfusion
Rat organs
Rats
Rats, Wistar
Sex Characteristics
Trace elements
Trace Elements - analysis
Trace Elements - blood
Macronutrients
Micronutrients
Rat organs
Gender differences
Trace elements
ICP-MS
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Summary:Concentrations of macro- and microelements in animal organs indicate the animal health status and represent reference data for animal experiments. Their levels in blood and tissues could be different between sexes, and could be different with and without blood in tissues. To test these hypotheses, in adult female and male rats the concentrations of various elements were measured in whole blood, blood plasma, and tissues from blood-containing (nonperfused) and blood-free liver, kidneys, and brain (perfused in vivo with an elements-free buffer). In these samples, 6 macroelements (Na, Mg, P, S, K, Ca) and 14 microelements (Fe, Mn, Co, Cu, Zn, Se, I, As, Cd, Hg, Pb, Li, B, Sr) were determined by inductively coupled plasma mass spectrometry following nitric acid digestion. In blood and plasma, female- or male-dominant sex differences were observed for 6 and 5 elements, respectively. In nonperfused organs, sex differences were observed for 3 (liver, brain) or 9 (kidneys) elements, whereas in perfused organs, similar differences were detected for 9 elements in the liver, 5 in the kidneys, and none in the brain. In females, perfused organs had significantly lower concentrations of 4, 5, and 2, and higher concentrations of 10, 4, and 7 elements, respectively, in the liver, kidneys, and brain. In males, perfusion caused lower concentrations of 4, 7, and 2, and higher concentrations of 1, 1, and 7 elements, respectively, in the liver, kidneys, and brain. Therefore, the residual blood in organs can significantly influence tissue concentrations of various elements and their sex-dependency.
ISSN:0946-672X
1878-3252
DOI:10.1016/j.jtemb.2016.12.015