Identification of novel microsatellite loci in the sand martin, Riparia riparia, and cross-amplification of loci from other bird species

We isolated and characterised six novel microsatellite loci for paternity analysis in the sand martin Riparia riparia , by screening an enriched genomic library. In addition, we tested 16 already published microsatellite markers, five of which were also polymorphic in the sand martin. Only one of th...

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Bibliographic Details
Published inJournal of ornithology Vol. 151; no. 3; pp. 761 - 764
Main Authors van Dongen, Wouter F. D., Munimanda, Gopi K., Augustin, Jakob, Blomqvist, Donald, Szép, Tibor, Wagner, Richard H.
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 2010
Springer Nature B.V
Subjects
Alleles
Ecology
Fish & Wildlife Biology & Management
Genetic markers
Heterozygosity
Life Sciences
Microsatellites
Paternity
Riparia riparia
Technical Note
Zoology
Microsatellite
Cross-amplification
Paternity
Sand Martin
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Summary:We isolated and characterised six novel microsatellite loci for paternity analysis in the sand martin Riparia riparia , by screening an enriched genomic library. In addition, we tested 16 already published microsatellite markers, five of which were also polymorphic in the sand martin. Only one of these 11 loci exhibited evidence of null alleles, and all were polymorphic (mean H o  = 0.68, range of number of alleles per locus = 4–24), making them suitable for individual heterozygosity quantification and paternity assessment in this species (exclusion probability of 11 unlinked loci = 0.999997).
ISSN:2193-7192
2193-7206
DOI:10.1007/s10336-010-0494-z