Androgenic dihaploids from somatic hybrids between Solanum melongena and S. aethiopicum group gilo as a source of resistance to Fusarium oxysporum f. sp. melongenae

• Published in: Plant cell reports Vol. 20; no. 11; pp. 1022 - 1032
• Main Authors: RIZZA, F, MENNELLA, G, COLLONNIER, C, SIHACHAKR, D, KASHYAP, V, RAJAM, M. V, PRESTERA, M, ROTINO, G. L
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.05.2002; Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Chloroplasts; Dihaploid; Flow cytometry; Fundamental and applied biological sciences. Psychology; Fusarium; Fusarium oxysporum; Genetic variability; Genetics and breeding of economic plants; Hybrids; Interspecific and intergeneric hybridization, introgressions; Plant breeding: fundamental aspects and methodology; Ploidy; Pollen; Solanum melongena; Viability; Wilt; Anther; Solanum melongena; Plant pathogen; Tissue culture; Introgression; Fungus resistance; Fungi; Androgenesis; Disease resistance; Sensitivity resistance; Vegetable crop; Dicotyledones; Morphology; Angiospermae; Genetic improvement; Somatic hybrid; Spermatophyta; Pollen; Fungi Imperfecti; Solanaceae; Viability; Thallophyta; Doubled haploid
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-001-0429-5

Dihaploid plants were obtained through anther culture of somatic hybrids between eggplant and Solanum aethiopicumgilo. The androgenic origin of the dihaploids was demonstrated by ploidy determination (flow cytometry and chloroplast counting) and isozyme and molecular (I-SSR and RAPDs) analyses. The androgenic plants showed significant morphological variability in the traits analysed. Pollen viability in the diploid androgenic plants was drastically reduced with respect to the somatic hybrids; however most of the dihaploids produced parthenocarpic fruits. S. aethiopicum and the somatic hybrids showed complete resistance to fungal wilt caused by Fusarium oxysporum f. sp. melongenae. Out of the 41 dihaploids inoculated, 34 were symptomless. The population of androgenic plants developed may represent a useful source for introgression of the Fusarium resistance trait into eggplant.