A haplotype at the UCP1 gene locus contributes to genetic risk for type 2 diabetes in Asian Indians (CURES-72)

• Published in: Metabolic syndrome and related disorders Vol. 8; no. 1; p. 63
• Main Authors: Vimaleswaran, Karani S, Radha, Venkatesan, Ghosh, Saurabh, Majumder, Partha P, Rao, M R S, Mohan, Viswanathan
• Format: Journal Article
• Language: English
• Published: United States 01.02.2010
• Subjects: Adipose Tissue - metabolism; Asian Continental Ancestry Group - genetics; Case-Control Studies; Diabetes Mellitus, Type 2 - diagnosis; Diabetes Mellitus, Type 2 - ethnology; Diabetes Mellitus, Type 2 - genetics; Female; Glucose - metabolism; Haplotypes; Humans; India; Ion Channels - genetics; Ion Channels - physiology; Male; Mitochondrial Proteins - genetics; Mitochondrial Proteins - physiology; Odds Ratio; Polymorphism, Genetic; Risk; Risk Factors; Uncoupling Protein 1
• ISSN: 1557-8518
• DOI: 10.1089/met.2009.0039

The gene encoding for uncoupling protein-1 (UCP1) is considered to be a candidate gene for type 2 diabetes because of its role in thermogenesis and energy expenditure. The objective of the study was to examine whether genetic variations in the UCP1 gene are associated with type 2 diabetes and its related traits in Asian Indians. The study subjects, 810 type 2 diabetic subjects and 990 normal glucose tolerant (NGT) subjects, were chosen from the Chennai Urban Rural Epidemiological Study (CURES), an ongoing population-based study in southern India. The polymorphisms were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Linkage disequilibrium (LD) was estimated from the estimates of haplotypic frequencies. The three polymorphisms, namely -3826A-->G, an A-->C transition in the 5'-untranslated region (UTR) and Met229Leu, were not associated with type 2 diabetes. However, the frequency of the A-C-Met (-3826A-->G-5'UTR A-->C-Met229Leu) haplotype was significantly higher among the type 2 diabetic subjects (2.67%) compared with the NGT subjects (1.45%, P < 0.01). The odds ratio for type 2 diabetes for the individuals carrying the haplotype A-C-Met was 1.82 (95% confidence interval, 1.29-2.78, P = 0.009). The haplotype, A-C-Met, in the UCP1 gene is significantly associated with the increased genetic risk for developing type 2 diabetes in Asian Indians.