Development of an SH-SAW sensor for the detection of DNA hybridization

We have developed SH (shear horizontal) surface acoustic wave (SAW) sensors for the detection of DNA (deoxyribonucleic acid) hybridization on the gold-coated delay line of transverse SAW devices. DNA hybridization experiments were performed with 15-mer oligonucleotides (probe and complementary targe...

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Bibliographic Details
Published inSensors and actuators. A. Physical. Vol. 120; no. 2; pp. 462 - 467
Main Authors Hur, Youngjune, Han, Jinho, Seon, Jooheon, Pak, Yukeun Eugene, Roh, Yongrae
Format Journal Article
LanguageEnglish
Published Elsevier B.V 17.05.2005
Subjects
DNA hybridization
DNA immobilization
Oligonucleotide
Oscillator
SH-SAW
DNA immobilization
Oligonucleotide
DNA hybridization
Oscillator
SH-SAW
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Summary:We have developed SH (shear horizontal) surface acoustic wave (SAW) sensors for the detection of DNA (deoxyribonucleic acid) hybridization on the gold-coated delay line of transverse SAW devices. DNA hybridization experiments were performed with 15-mer oligonucleotides (probe and complementary target DNA). The sensor consists of twin SAW delay line oscillators (sensing channel and reference channel) operating at 100 MHz fabricated on 36° rotated Y-cut X-propagation LiTaO 3 piezoelectric single crystals. The relative change in the frequency of the two oscillators was monitored to detect hybridization between the target DNA and probe DNA immobilized on the delay line of the SAW sensor. The measurement results showed a good response of the sensor to the mass loading effects of the DNA hybridization with a sensitivity level up to 1.55 ng/ml/Hz.
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ISSN:0924-4247
1873-3069
DOI:10.1016/j.sna.2005.01.027