Effect of biological pretreatment with Trametes hirsuta yj9 on enzymatic hydrolysis of corn stover

In this study, a newly isolated Trametes hirsuta yj9 was used to pretreat corn stover in order to enhance enzymatic digestibility. T. hirsuta yj9 preferentially degraded lignin to be as high as 71.49% after 42-day pretreatment. Laccase and xylanase was the major ligninolytic and hydrolytic enzyme, r...

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Bibliographic Details
Published inInternational biodeterioration & biodegradation Vol. 65; no. 7; pp. 931 - 938
Main Authors Sun, Feng-hui, Li, Jiang, Yuan, Yue-xiang, Yan, Zhi-ying, Liu, Xiao-feng
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.10.2011
Subjects
Biodegradation
Biological pretreatment
cellulose
cellulosic fibers
Coriolus hirsutus
Corn stover
Enzymatic hydrolysis
hemicellulose
laccase
Lignin
scanning electron microscopy
sugars
Trametes hirsuta
Trametes hirsuta yj9
xylanases
yields
Lignin
Corn stover
Enzymatic hydrolysis
Trametes hirsuta yj9
Biological pretreatment
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Summary:In this study, a newly isolated Trametes hirsuta yj9 was used to pretreat corn stover in order to enhance enzymatic digestibility. T. hirsuta yj9 preferentially degraded lignin to be as high as 71.49% after 42-day pretreatment. Laccase and xylanase was the major ligninolytic and hydrolytic enzyme, respectively and filter paper activity (FPA) increased gradually with prolonged pretreatment time. Sugar yields increased significantly after pretreatment with T. hirsuta yj9, reaching an enzymatic digestibility of 73.99% after 42 days of pretreatment. Scanning electron microscopy (SEM) showed significant structural changes in pretreated corn stover, the surface of pretreated corn stover became increasingly coarse, the gaps between cellulose fibers were visible, and many pores were developed. Correlation analysis showed that sugar yields were inversely proportional to the lignin contents, less related to cellulose and hemicellulose contents. ► Trametes hirsuta yj9 preferentially degrade lignin over cellulose. ► Laccase is the major ligninolytic enzyme. ► Sugar yields of pretreated corn stover were significantly increased. ► The structure of pretreated corn stover showed significant changes. ► Sugar yields were inversely proportional to the lignin contents.
Bibliography:http://dx.doi.org/10.1016/j.ibiod.2011.07.001
ObjectType-Article-2
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ISSN:0964-8305
1879-0208
DOI:10.1016/j.ibiod.2011.07.001