Molecular systematics of Goodyerinae (Cranichideae, Orchidoideae, Orchidaceae) based on multiple nuclear and plastid regions

• Published in: Molecular phylogenetics and evolution Vol. 139; p. 106542
• Main Authors: Chen, Shi-Pin, Tian, Huai-Zhen, Guan, Qiu-Xiang, Zhai, Jun-Wen, Zhang, Guo-Qiang, Chen, Li-Jun, Liu, Zhong-Jian, Lan, Si-Ren, Li, Ming-He
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2019
• Subjects: Bayes Theorem; Cell Nucleus - genetics; Cheirostylidinae; Jewel orchid; Ludisiinae; Orchidaceae - classification; Orchidaceae - genetics; Pachyplectroninae; Phylogeny; Physurinae; Plastids - genetics; Terrestrial orchids; Physurinae; Ludisiinae; Pachyplectroninae; Cheirostylidinae; Terrestrial orchids; Jewel orchid
• ISSN: 1055-7903; 1095-9513
• DOI: 10.1016/j.ympev.2019.106542

[Display omitted] •We examined the first comprehensive molecular phylogenetics of Goodyerinae based on seven markers and a morphological discussion.•Phylogenetic analyses indicated that Goodyerinae can be grouped into three distinct lineages.•Erythrodes, Goodyera, Myrmechis and Odontochilus are polyphyletic, as currently circumscribed. Goodyerinae are one of the most species-rich and widespread subtribes of Orchidaceae but notorious for their taxonomic difficulty. Here, a comprehensive molecular phylogenetic study of the subtribe is presented based on two nuclear (ITS, Xdh) and five plastid (matK, psaB, rbcL, trnL, trnL-F) regions. A total of 119 species were included representing all clades recovered by previous phylogenetic analyses as well as seven outgroups. Maximum parsimony, maximum likelihood and Bayesian inference methods were used to infer the phylogenetic relationships. The results show that the Goodyerinae subdivided into three major subdivisions and six groupings: Pachyplectron, Goodyera clade (including Goodyera procera, Microchilus subclade and Goodyera subclade) and Cheirostylis clade (including Gonatostylis, Cheirostylis subclade and Ludisia subclade). Four genera, Erythrodes, Goodyera, Myrmechis and Odontochilus, are not monophyletic. The results support Odontochilus s. l. to include Myrmechis and Kuhlhasseltia. The systematic positions of Goodyera procera and two isolated genera, Herpysma and Orchipedum, are difficult to determine.