Biodegradation of photo-degraded mulching films based on polyethylenes and stearates of calcium and iron as pro-oxidant additives

• Published in: International biodeterioration & biodegradation Vol. 65; no. 3; pp. 451 - 459
• Main Authors: Abrusci, C., Pablos, J.L., Corrales, T., López-Marín, J., Marín, I., Catalina, F.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.06.2011
• Subjects: additives; Agriculture; Bacillus; Bacillus cereus; Bacillus megaterium; Bacteria; bioassays; Biodegradation; biofilm; Brevibacillus; Brevibacillus borstelensis; buried soils; calcium; carbon dioxide; chemiluminescence; data analysis; emissions; Fourier transform infrared spectroscopy; genes; impedance; iron; irradiation; microorganisms; mineralization; mulching; Mulching films; oxidation; Polyethylene; polyethylene film; polymerase chain reaction; Pro-oxidants; ribosomal DNA; ribosomal RNA; temperature; Spain; Spain, Murcia; Biodegradation; Bacteria; Polyethylene; Agriculture; Pro-oxidants; Mulching films
• ISSN: 0964-8305; 1879-0208
• DOI: 10.1016/j.ibiod.2010.10.012

Polyethylene film materials persist in the environment for a long time. Several bacterial species have been isolated from films buried in soil located in Murcia, Spain. Bacterial strains were characterized with a combination of culture-dependent methods and sequencing of part of the 16S ribosomal RNA gene (rDNA) after amplification by polymerase chain reaction (PCR). Three bacterial species common in soil were found attached to the polymer and identified as Bacillus. cereus, B. megaterium, and B. subtilis. These microorganisms, as well as Brevibacillus borstelensis, were tested for biodegradation susceptibility at 30 and 45 °C on highly photo-degraded polyethylene films (500 h under irradiation of Xe-Lamp-solar filter) that contained calcium and iron stearates as pro-oxidant additives. Biofilm formation developed on the photo-degraded materials after one week of bacterial treatment. Biodegradation of the polyethylene films was studied by chemiluminescence, ATR–FTIR, and GC-product analysis and the data confirm a more efficient biodegradation on the bioassays carried out at higher temperature. The CL emissions due to decomposition of oxidation species take place at lower temperatures; the decrease of carbonyl index and the disappearance of photogenerated low-molecular products with biodegradation were more efficient on the biodegraded films at 45 °C. Also, mineralization was evaluated by carbon dioxide measurements using an indirect impedance technique. Biodegradation by B. borstelensis and MIX at 30 °C was slow and in the range of 0.7–1.2% of mineralization after 90 days of bacterial bioassay. At 45 °C biodegradation was more efficient and in particular in the more photo-degraded films containing Ca and Fe stearates where mineralization extents reached values of 11.5% with B. borstelensis and 7–10% with the mixture of Bacillus (MIX).