Determining the effectiveness of using dermal swabs to evaluate the stress physiology of laboratory cephalopods: A preliminary investigation

• Published in: General and comparative endocrinology Vol. 314; p. 113903
• Main Authors: Chancellor, Stephanie, Abbo, Lisa, Grasse, Bret, Sakmar, Taylor, Brown, Joel S., Scheel, David, Santymire, Rachel M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2021
• Subjects: Adrenocorticotropic Hormone; Animals; Biomarkers of stress; Cephalopoda; Corticosterone; Cortisol; Euprymna berryi; Humans; Hydrocortisone; Laboratories; Minimally-invasive skin swab; Octopus chierchiae; Octopus chierchiae; Minimally-invasive skin swab; Cortisol; Biomarkers of stress; Corticosterone; Euprymna berryi
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2021.113903

•Use of skin mucus swabs to measure immunoreactive glucocorticoids (cortisol and corticosterone) for three species of cephalopods.•These results demonstrate the first instance where cortisol is measured in a cephalopod.•Stress hormones fluctuate when introduced to a new habitat.•Younger individuals have higher cortisol concentrations compared to older individuals. To better comprehend the physiology of cephalopods, we used a minimal invasive technique of skin mucus swabs to measure immunoreactive corticosteroids in three cephalopod species commonly kept in captivity and promoted as new model organisms: Euprymna berryi, Sepia bandensis, and Octopus chierchiae. We compared results between sexes and age classes and then evaluated their stress responses during acclimation to a new habitat. To better understand glucocorticoid production, we conducted an adrenocorticotropic hormone, using Cosyntropin (an adrenocorticotropin (ACTH) analogue) challenge with a saline control and swabbed their mantles at 15-minute intervals for 2 h. Results showed cortisol was higher for younger individuals. Additionally, cortisol and corticosterone concentrations decreased by 2-fold after 2 to 4 days of acclimation to a new habitat. We were able to successfully measure 2-fold increase in immunoreactive corticosteroids which reacted with cortisol and corticosterone assays for all the species following ACTH injection, although not all individuals responded similarly. With further investigation, this technique can increase our understanding and management of cephalopods in captivity.