Glutathione-Dependent Cell Cycle G1 Arrest and Apoptosis Induction in Human Lung Cancer A549 Cells Caused by Methylseleninic Acid: Comparison with Sodium Selenite

• Published in: Biological & pharmaceutical bulletin Vol. 37; no. 11; pp. 1831 - 1837
• Main Authors: Okuno, Tomofumi, Honda, Eri, Arakawa, Tomohiro, Ogino, Hirofumi, Ueno, Hitoshi
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 2014
• Subjects: Antineoplastic Agents - pharmacology; apoptosis; Apoptosis - drug effects; cell cycle arrest; Cell Line, Tumor; G1 Phase Cell Cycle Checkpoints - drug effects; Gene Expression Profiling; Glutathione - metabolism; human lung cancer A549 cell; Humans; Lung Neoplasms - metabolism; methylseleninic acid; Organoselenium Compounds - pharmacology; reduced glutathione; Sodium Selenite - pharmacology
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.b14-00453

The aim of the present study was to clarify the mechanism underlying the inhibition of cell proliferation in human lung cancer A549 cells by selenium (Se) compounds. Methylseleninic acid (CH3SeO2H, abbreviated as MSA), a synthetic Se compound, is a direct precursor of active methylselenol (CH3SeH) and is considered to be one of beneficial agents for cancer prevention and therapy. Sodium selenite (Na2SeO3), an inorganic Se form, is utilized in clinical Se supplementation. MSA markedly inhibited the growth of A549 cells at a concentration of 2.5×10−6 mol/L for 1 d. On Day 1, Na2SeO3 also inhibited A549 cell growth at the concentration of 7.5×10−6 mol/L. These compounds induced cell cycle arrest at the G1 phase and apoptosis under the inhibitory condition. Reduced glutathione (GSH) is critical to MSA or Na2SeO3 metabolism. The depletion of intracellular GSH suppressed Na2SeO3-induced G1 arrest, but promoted Na2SeO3-induced apoptosis. Therefore, Na2SeO3 appears to have directly induced apoptosis. In contrast, the MSA-induced G1 arrest was ameliorated by a marked decrease in GSH content. Additionally, the depletion of GSH slightly suppressed MSA-induced apoptosis. The difference in inhibitory effects between MSA and Na2SeO3 may be due to this variation in GSH-related metabolism. After exposure of A549 cells to MSA, the GSH content was significantly decreased. These results indicate that because MSA-induced G1 arrest and apoptosis induction are enhanced by GSH, the maintenance of GSH is essential for the effective anticancer action of MSA in A549 cells.