The C4280A (rs5705) gene polymorphism of the renin (REN) gene is associated with risk of developing coronary artery disease, but not with restenosis after coronary stenting

• Published in: Experimental and molecular pathology Vol. 99; no. 1; pp. 128 - 132
• Main Authors: Fragoso, Jose Manuel, Alvarez-León, Edith, Delgadillo-Rodríguez, Hilda, Arellano-González, Marva, López-Pacheco, Filogonio Caín, Cruz-Robles, David, Peña-Duque, Marco Antonio, Pérez-Méndez, Oscar, Martínez-Ríos, Marco Antonio, Vargas-Alarcón, Gilberto
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.08.2015
• Subjects: Aged; Alleles; Angiotensinogen - genetics; Angiotensinogen - metabolism; Case-Control Studies; Computational Biology; Coronary artery disease; Coronary Artery Disease - genetics; Coronary Restenosis - genetics; Female; Gene Frequency; Genetic Markers; Genetic Predisposition to Disease; Genotyping Techniques; Haplotypes; Heterozygote; Humans; Linkage Disequilibrium; Logistic Models; Male; Mexico; Middle Aged; Polymorphism, Single Nucleotide; Polymorphisms; Renin - genetics; Renin - metabolism; Renin–angiotensin-system (RAS); Restenosis; Risk Factors; Stents; Mexico; Restenosis; Polymorphisms; Renin–angiotensin-system (RAS); Coronary artery disease
• ISSN: 0014-4800; 1096-0945
• DOI: 10.1016/j.yexmp.2015.06.012

The aim of the present study was to evaluate the role of AGT and REN gene polymorphisms as susceptibility markers for coronary artery disease (CAD) and/or restenosis after coronary stent placement in a group of Mexican patients. Five polymorphisms of the AGT (rs699, rs4762, rs5051, rs5049, rs5046) and two of the REN (rs5707, rs5705) genes were analyzed by 5′ exonuclease TaqMan genotyping assays in 240 patients with CAD who underwent coronary artery stenting (76 with restenosis and 164 without restenosis). A group of 610 individuals without clinical and familial antecedents of cardiovascular diseases were included as controls. The results showed that the distribution of AGT and REN polymorphisms were similar in patients with and without restenosis. However, when the whole group of patients (with and without restenosis) was compared to healthy controls, under co-dominant, dominant, heterozygous and additive models, the REN A4280C (rs5705) polymorphism was associated with increased risk of CAD (OR=1.76, PCo-dom=0.006, OR=1.81, PDom=0.001, OR=1.75, PHet=0.003 and OR=1.59, PAdd=0.003, respectively). All models were adjusted for age, gender, diabetes, dyslipidemia, hypertension and smoking habit. The TC haplotype of the REN gene was associated with increased risk of CAD (OR=1.53, P=0.014). The data suggest that the REN C4280A (rs5705) polymorphism plays an important role in the risk of developing CAD with the highest risk for C allele, but do not support its role as a risk factor for developing restenosis after coronary stenting. •REN C4280A (rs5705) polymorphism plays an important role in the risk of developing CADE.•The results do not support the role of REN polymorphisms as a risk factor for developing restenosis after coronary stenting.•One REN haplotype was associated with risk of developing CAD•A functional effect of the C4280A (rs5705) polymorphism is suggested by bioinformatics tools.