Molecular sexing of tigers, Panthera tigris

We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is use...

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Bibliographic Details
Published inConservation genetics resources Vol. 4; no. 2; pp. 299 - 301
Main Authors McEwing, R., Ouitavon, K., Rovie-Ryan, J. J., Wulansari, Sitam, F. T., Ogden, R.
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.06.2012
Springer Nature B.V
Subjects
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Capillary electrophoresis
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Gene polymorphism
Life Sciences
Plant Genetics and Genomics
Sexing
Technical Note
Zinc finger proteins
Zinc finger protein
Forensics
Molecular sex identification
Tiger
Felids
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Summary:We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-011-9529-x