Nocardioides daecheongensis sp. nov., isolated from soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 64; no. Pt 12; pp. 4109 - 4114
• Main Authors: Lim, Jun-Muk, Kim, Soo-Jin, Hamada, Moriyuki, Ahn, Jae-Hyung, Weon, Hang-Yeon, Suzuki, Ken-ichiro, Ahn, Tae-Young, Kwon, Soon-Wo
• Format: Journal Article
• Language: English
• Published: England International Union of Microbiological Societies 01.12.2014
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Bacterial Typing Techniques; Base Composition; Diaminopimelic Acid - chemistry; DNA; DNA, Bacterial - genetics; fatty acid composition; Fatty Acids - chemistry; Forests; lipids; microbiology; Molecular Sequence Data; Nocardioides; Nucleic Acid Hybridization; nucleotide sequences; Peptidoglycan - chemistry; peptidoglycans; Phylogeny; Republic of Korea; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; soil; Soil Microbiology; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; South Korea
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.063610-0

Strain KIS2-16 ᵀ was isolated from a soil sample collected from Daecheong Island of Incheon region, South Korea. KIS2-16 ᵀ was Gram-staining-positive, aerobic, non-spore-forming, non-motile, catalase-positive, oxidase-negative and mesophilic. On the basis of 16S rRNA gene sequence analysis, strain KIS2-16 ᵀ represented a member of the genus Nocardioides , being most closely related to the type strains of species of the genus Nocardioides , Nocardioides maradonensis RP-B30 ᵀ (97.8 % sequence similarity) and Nocardioides ultimimeridianus RP-B26 ᵀ (97.0 %). The fatty acid profile of KIS2-16 ᵀ was dominated by C ₁₈ : ₁ω9 c , C ₁₇ : ₁ω8 c , C ₁₆ : ₀, C ₁₈ : ₀ 10-methyl (TBSA), C ₁₆ : ₀ 2-OH and C ₁₇ : ₀ 2-OH. The major isoprenoid quinone was MK-8(H ₄), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The peptidoglycan structure was A3γ-type with ll -diaminopimelic acid. The genomic DNA G+C content of KIS2-16 ᵀ was 64.9 mol%. Strain KIS2-16 ᵀ showed DNA–DNA hybridization values of less than 70 % with the closely related species of the genus Nocardioides . Based on phenotypic, genotypic and phylogenetic data, the isolate represents a novel species of the genus Nocardioides , for which the name Nocardioides daecheongensis sp. nov. (type strain KIS2-16 ᵀ = DSM 27136 ᵀ = KACC 17297 ᵀ = NBRC 109597 ᵀ) is proposed.