High-performance liquid chromatographic approach to the separation of antiviral and immunostimulant fractions in Neuramide

Neuramide, a tissue extract having antiviral action against influenza A virus and immunostimulant action, was analyzed by preparative size-exclusion high-performance liquid chromatography (HPLC) and a low-molecular-weight fraction responsible for the antiviral action was isolated after reversed-phas...

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Bibliographic Details
Published inJournal of chromatography Vol. 512; p. 123
Main Authors Miraglia, N, Rindone, B, Folchitto, G, Amicucci, P, Antonelli, G, Lanfranchi, A, Massa, M
Format Journal Article
LanguageEnglish
Published Netherlands 20.07.1990
Subjects
Adjuvants, Immunologic - isolation & purification
Antimicrobial Cationic Peptides
Antiviral Agents - isolation & purification
Cell Division - drug effects
Cells, Cultured
Chromatography, High Pressure Liquid
Humans
In Vitro Techniques
Influenza A virus - drug effects
Leukocytes, Mononuclear - drug effects
Lymphocytes - drug effects
Methylene Chloride
Molecular Weight
Peptides - analysis
Peptides - chemistry
Peptides - pharmacology
Ultrafiltration
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Summary:Neuramide, a tissue extract having antiviral action against influenza A virus and immunostimulant action, was analyzed by preparative size-exclusion high-performance liquid chromatography (HPLC) and a low-molecular-weight fraction responsible for the antiviral action was isolated after reversed-phase HPLC. Four fractions having immunostimulant activity were also isolated, as evidenced by their potentiating action in the human lymphocyte proliferation induced by phytohaemagglutinin.
DOI:10.1016/S0021-9673(01)89478-4