Hemagglutination variant of measles virus

A hemagglutination variant (HAN-12) of measles virus was isolated following mutagenesis of the parental strain (CC). Cells infected with HAN-12 formed syncytia but did not adsorb monkey erythrocytes. Although HAN-12 produced normal yields of infectious virus, no hemagglutination or hemolysis activit...

Full description

Saved in:
Bibliographic Details
Published inVirology (New York, N.Y.) Vol. 80; no. 2; pp. 441 - 444
Main Authors Breschkin, Alan M., Walmer, Barbara, Rapp, Frde
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.07.1977
Subjects
Animals
Cricetinae
Erythrocytes - immunology
Genetic Variation
Haplorhini
Hemadsorption
Hemagglutination, Viral
Hemolysis
Hypertonic Solutions
Measles virus - analysis
Measles virus - immunology
Measles virus - pathogenicity
Mesocricetus
Mutagens
Nitrosoguanidines
Viral Proteins - analysis
Virulence
Online AccessGet full text

Cover

More Information
Summary:A hemagglutination variant (HAN-12) of measles virus was isolated following mutagenesis of the parental strain (CC). Cells infected with HAN-12 formed syncytia but did not adsorb monkey erythrocytes. Although HAN-12 produced normal yields of infectious virus, no hemagglutination or hemolysis activity was detected when assayed in isotonic phosphate-buffered saline. However, in hypertonic buffer the hemagglutination and hemolysis titers of HAN-12 were equivalent to that of CC. In isotonic medium, HAN-12 adsorbed to host cells as efficiently as the parental strain. Comparison of HAN-12 and CC virion-associated polypeptides by SDS-polyacrylamide gel electrophoresis did not reveal any differences in migration rates of the proteins. Our results are discussed in regard to previous reports of hemagglutination variants of measles virus.
ISSN:0042-6822
1096-0341
DOI:10.1016/S0042-6822(77)80021-4