Up-regulation of B-cell lymphoma factor-2 expression, inhibition of oxidative stress and down-regulation of pro-inflammatory cytokines are involved in the protective effect of cabbage (Brassica oleracea) juice in lead-induced endothelial dysfunction in rats

• Published in: Journal of trace elements in medicine and biology Vol. 73; p. 127014
• Main Authors: Asiwe, Jerome Ndudi, Kolawole, Tolunigba Abisola, Ben-Azu, Benneth, Ajayi, Abayomi Mayowa, Ojetola, Abodunrin Adebayo, Moke, Emuesiri Goodies, Nwangwa, Eze Kingsley
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.09.2022
• Subjects: Cabbage juice; Endothelial dysfunction; Lead acetate; Oxido-inflammatory stress; Vasoconstrictor; Vasodilator; Endothelial dysfunction; Cabbage juice; Lead acetate; Oxido-inflammatory stress; Vasoconstrictor; Vasodilator
• ISSN: 0946-672X; 1878-3252
• DOI: 10.1016/j.jtemb.2022.127014

Oxido-inflammatory stress and dysregulation of nitric oxide (NO) system has been implicated in lead toxicity. Cabbage is an antioxidant-rich household vegetable with plethora of therapeutic potentials. The present study investigated the anti-oxido-inflammatory activity of cabbage in lead-induced endothelial dysfunction. Twenty (20) male Wistar rats were selected into four groups (n = 5) and treated with distilled water (1 mL/100 g b.wt), lead acetate (25 mg/kg b.wt), cabbage juice (1 mL/100 g b.wt) and lead acetate (25 mg/kg b.wt) plus cabbage juice (1 mL/100 g b.wt) respectively. Treatment was done orally for 28 days, thereafter, oxidative stress (SOD, CAT, GSH, and MDA), inflammatory (TNF-α and IL-6) and apoptotic (Bcl-2) markers were assayed using standard biochemical assays as well as histoarchitectural study of the endothelium. The results showed that they were significant increase in MDA, ET-1, TNF-α and IL-6 while SOD, GSH, CAT, NO and Bcl-2 protein expression were decreased in Lead exposed animals. Endothelial histoarchitecture was also altered. Following Cabbage juice treatment, MDA, ET-I, TNF-α and IL-6 were down-regulated while SOD, GSH, CAT, NO and Bcl-2 protein expression were up-regulated. Histoarchitecture was significantly recovered. The study suggests that cabbage juice could mitigates Lead-induced endothelial dysfunction by modulating oxido-inflammatory and anti-apoptotic mediators. All data are available upon request.