Restriction fragment length polymorphism analysis of isolates of infectious bursal disease viruses from Turkey

The aim of this study was to investigate the genetic variation among infectious bursal disease virus (IBDV) isolates from commercially reared chickens in Elazig province (Turkey) with the use of restriction enzyme Mbol in RT/PCR-RFLP. A total of 40 IBDV positive bursa samples were used. The assay am...

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Published inVeterinární medicína Vol. 48; no. 12; pp. 359 - 362
Main Authors Ozbey, G, Ertas, H.B, Muz, A. (University of Firat, Elazig (Turkey). Dept. of Microbiology)
Format Journal Article
LanguageEnglish
Published Czech Academy of Agricultural Sciences 01.12.2003
Subjects
commercially reared chickens
DINDON
ENFERMEDAD DE GUMBORO
GENES
GENETIC VARIATION
GUMBORO DISEASE
GÈNE
ibdv
MALADIE DE GUMBORO
PAVO
PCR
RFLP
rt/pcr
TURKEYS
VARIACIÓN GENÉTICA
VARIATION GÉNÉTIQUE
VIRUS
VIRUSES
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Abstract The aim of this study was to investigate the genetic variation among infectious bursal disease virus (IBDV) isolates from commercially reared chickens in Elazig province (Turkey) with the use of restriction enzyme Mbol in RT/PCR-RFLP. A total of 40 IBDV positive bursa samples were used. The assay amplified a 743 bp fragment of the IBDV VP2 gene. Most of the viruses had the same RFLP with the MboI enzyme. RFLP analysis of the isolates produced two different band profiles. The results of this study showed that little genetic heterogeneity existed among IBDV strains in the infected flock.
AbstractList The aim of this study was to investigate the genetic variation among infectious bursal disease virus (IBDV) isolates from commercially reared chickens in Elazig province (Turkey) with the use of restriction enzyme Mbol in RT/PCR-RFLP. A total of 40 IBDV positive bursa samples were used. The assay amplified a 743 bp fragment of the IBDV VP2 gene. Most of the viruses had the same RFLP with the MboI enzyme. RFLP analysis of the isolates produced two different band profiles. The results of this study showed that little genetic heterogeneity existed among IBDV strains in the infected flock.
Infectious bursal disease Virus (IBDV) specific reverse transcriptase/polymerase chain recation (RT/PCR) positive 40 broiler bursa fabricius samples obtained from a commercially reared flock were investigated for genetic diversity by PCR-RFLP assay. The assay amplifies a 743 bp fragment of the IBDV VP2 gene. The RFLP profiles of 40 of these positive samples were determined using the enzyme MboI. Most of the viruses had the same RFLP with the MboI enzyme. RFLP analysis of the isolates produced two different band profiles. The results of this study showed that little genetic heterogeneity exists among IBDV strains in a infected flock.
ArticleNumber 359
Author Muz, A. (University of Firat, Elazig (Turkey). Dept. of Microbiology)
Ertas, H.B
Ozbey, G
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Infectious bursal disease Virus (IBDV) specific reverse transcriptase/polymerase chain recation (RT/PCR) positive 40 broiler bursa fabricius samples obtained...
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StartPage 359
SubjectTerms commercially reared chickens
DINDON
ENFERMEDAD DE GUMBORO
GENES
GENETIC VARIATION
GUMBORO DISEASE
GÈNE
ibdv
MALADIE DE GUMBORO
PAVO
PCR
RFLP
rt/pcr
TURKEYS
VARIACIÓN GENÉTICA
VARIATION GÉNÉTIQUE
VIRUS
VIRUSES
Title Restriction fragment length polymorphism analysis of isolates of infectious bursal disease viruses from Turkey
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