Restriction fragment length polymorphism analysis of isolates of infectious bursal disease viruses from Turkey

The aim of this study was to investigate the genetic variation among infectious bursal disease virus (IBDV) isolates from commercially reared chickens in Elazig province (Turkey) with the use of restriction enzyme Mbol in RT/PCR-RFLP. A total of 40 IBDV positive bursa samples were used. The assay am...

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Bibliographic Details
Published inVeterinární medicína Vol. 48; no. 12; pp. 359 - 362
Main Authors Ozbey, G, Ertas, H.B, Muz, A. (University of Firat, Elazig (Turkey). Dept. of Microbiology)
Format Journal Article
LanguageEnglish
Published Czech Academy of Agricultural Sciences 01.12.2003
Subjects
commercially reared chickens
DINDON
ENFERMEDAD DE GUMBORO
GENES
GENETIC VARIATION
GUMBORO DISEASE
GÈNE
ibdv
MALADIE DE GUMBORO
PAVO
PCR
RFLP
rt/pcr
TURKEYS
VARIACIÓN GENÉTICA
VARIATION GÉNÉTIQUE
VIRUS
VIRUSES
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Summary:The aim of this study was to investigate the genetic variation among infectious bursal disease virus (IBDV) isolates from commercially reared chickens in Elazig province (Turkey) with the use of restriction enzyme Mbol in RT/PCR-RFLP. A total of 40 IBDV positive bursa samples were used. The assay amplified a 743 bp fragment of the IBDV VP2 gene. Most of the viruses had the same RFLP with the MboI enzyme. RFLP analysis of the isolates produced two different band profiles. The results of this study showed that little genetic heterogeneity existed among IBDV strains in the infected flock.
Bibliography:2004000333
L73
ISSN:0375-8427
1805-9392
DOI:10.17221/5790-VETMED