Reference values of leukocyte and lymphocytes populations in umbilical cord and capillary blood in healthy Mexican newborns

• Published in: Allergologia et immunopathologia Vol. 48; no. 3; p. 295
• Main Authors: Scheffer-Mendoza, S, Espinosa-Padilla, S E, López-Herrera, G, Mujica-Guzmán, F, López-Padilla, M G, Berrón-Ruiz, L
• Format: Journal Article
• Language: English
• Published: Spain 01.05.2020
• Subjects: Cell Separation; Female; Fetal Blood - immunology; Flow Cytometry; Humans; Infant, Newborn; Leukocyte Count; Leukocytes - pathology; Lymphocyte Count; Lymphocyte Subsets - pathology; Lymphocytes - pathology; Male; Mexico; Reference Values; Mexico; Lymphocyte populations; Reference value; Newborn; Leukocytes; Umbilical cord blood; Capillary blood
• ISSN: 1578-1267
• DOI: 10.1016/j.aller.2019.12.009

In newborns, dramatic changes occur in the blood and bone marrow during the first hours; there are rapid fluctuations in the quantities of leukocytes populations. In this work, we investigated leukocytes subsets counts in two types of blood samples (cord blood and capillary blood) extracted from healthy newborns. Blood samples from Mexican neonates were collected by Instituto Nacional de Pediatría with written informed consent. For all samples we determined leukocytes populations; neutrophils, monocytes, total lymphocytes, and populations: T CD3+ cells, TCD4+ cells, T CD8+ cells, B CD19+ cells and NK CD16+56 cells by flow cytometry. We used the Mann-Whitney U test to compare leukocytes of cord and capillary blood; also to analyze the differences between gender and we obtained reference values of the cord and capillary blood in neonates. We observed higher absolute counts and frequencies of total lymphocyte in capillary blood compared with cord blood. In absolute numbers, the capillary blood showed significant differences in neutrophils, monocytes, lymphocytes, T CD3+ cells, T CD4+ cells, T CD8+ cells, B CD19+ cells, and NK cells; no significant differences were observed between genders. Our data contribute to newborn Mexican reference values for all these populations of leukocytes. We found that the dispersal range differs between the two types of blood, suggesting a different fate in the immune response. Immunophenotyping of the blood cell population to identify these cells is an essential tool in the diagnosis and follow-up of neonates with immunodeficiencies and other immune disorders.