Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy

• Published in: Microelectronic engineering Vol. 87; no. 5; pp. 802 - 805
• Main Authors: Kitsara, M., Petrou, P., Kontziampasis, D., Misiakos, K., Makarona, E., Raptis, I., Beltsios, K.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.2010; Elsevier
• Subjects: Applied sciences; Atomic force microscopy; Biomolecular layer; Biomolecules; Condensed matter: electronic structure, electrical, magnetic, and optical properties; Condensed matter: structure, mechanical and thermal properties; Electronics; Exact sciences and technology; Fullerenes and related materials; Gamma-globulins; Methodology; Optical constants: refractive index, complex dielectric constant, absorption, reflection and transmission coefficients, emissivity; Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation; Optical properties of bulk materials and thin films; Physics; Reflectance; Reflectance spectroscopy; Reflectivity; Solid surfaces; Solid surfaces and solid-solid interfaces; Spectroscopy; Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties); Testing, measurement, noise and reliability; Thin films; Visible and ultraviolet spectra; White light; Reflectance spectroscopy; Atomic force microscopy; Gamma-globulins; Biomolecular layer; Thin films; Thickness; Performance evaluation; White light; Reflection spectrum; Adsorption; Semiconductor device reliability
• ISSN: 0167-9317; 1873-5568
• DOI: 10.1016/j.mee.2009.11.082

A White Light Reflectance Spectroscopy (WLRS) methodology is applied in the evaluation of the effectiveness of biomolecules immobilization onto solid surfaces as well as their subsequent reaction with counterpart biomolecules via measuring the respective layers thickness. In particular we investigated the adsorption of rabbit and mouse gamma-globulins as well as their reaction with complementary antibodies. The results obtained with the proposed methodology were compared with those received by atomic force microscopy analysis of the same samples. It was found that the developed method provides a simple, very fast and accurate approach for thin biomolecular layers thickness determination.