Comparison of CK-MB fractionation by antibody inhibition reaction and DEAE-Sephadex chromatography

1. CK-MB isoenzyme fractionation has been compared between DEAE-sephadex chromatography and the CK-M-subunit inhibiting antibody procedure. 2. It was found that the inhibiting antibody method generally gave lower values than the chromatography procedure. 3. The above difference can be partially attr...

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Bibliographic Details
Published inClinical biochemistry Vol. 11; no. 4; pp. 175 - 178
Main Authors Itiaba, K., Crawhall, J.C.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.1978
Subjects
Antibodies
Binding, Competitive
Chromatography, Ion Exchange
Creatine Kinase - blood
Creatine Kinase - immunology
Humans
Isoenzymes - blood
Isoenzymes - immunology
Methods
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Summary:1. CK-MB isoenzyme fractionation has been compared between DEAE-sephadex chromatography and the CK-M-subunit inhibiting antibody procedure. 2. It was found that the inhibiting antibody method generally gave lower values than the chromatography procedure. 3. The above difference can be partially attributed to inhibitory factors in the antibody system. 4. It was concluded that although there was general agreement between the two procedures, the CK-M-subunit inhibiting antibody procedure should be viewed with caution at low creatine kinase activities, and should never be done without the appropriate reagent blanks.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-9120
1873-2933
DOI:10.1016/S0009-9120(78)90451-4