Sanguisorba officinalis L. Extracts Exert Antiobesity Effects in 3T3-L1 Adipocytes and C57BL/6J Mice Fed High-Fat Diets

• Published in: Journal of medicinal food Vol. 19; no. 8; p. 768
• Main Authors: Jung, Da-Woon, Lee, Ok-Hwan, Kang, Il-Jun
• Format: Journal Article
• Language: English
• Published: United States 01.08.2016
• Subjects: 3T3-L1 Cells; Adipocytes - metabolism; Adipogenesis; Adiponectin - blood; Adipose Tissue - metabolism; Animals; Anti-Obesity Agents - pharmacology; Anti-Obesity Agents - therapeutic use; CCAAT-Enhancer-Binding Protein-alpha - blood; Diet, High-Fat; Lipid Metabolism - drug effects; Male; Mice; Mice, Inbred C57BL; Mice, Obese; Obesity - blood; Obesity - etiology; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; PPAR gamma - blood; Sanguisorba; Sterol Regulatory Element Binding Protein 1 - blood; Transcription Factors - blood; Triglycerides - blood; Weight Loss - drug effects; Sanguisorba officinalis L; 3T3-L1 adipocytes; adipogenesis; antiobesity; lipolysis
• ISSN: 1557-7600
• DOI: 10.1089/jmf.2016.3704

The purpose of this study was to investigate the antiobesity effect of Sanguisorba officinalis L. (SOL) in 3T3-L1 adipocytes and obese C57BL/6J mice. SOL was extracted with water and 30%, 50%, 70%, and 100% ethanol (EtOH). 3T3-L1 adipocytes were treated with SOL extracts (100 μg/mL) during the differentiation period. Triglyceride (TG) accumulation was determined by Oil Red O staining, and the expression of adipocyte-specific proteins was measured by Western blot analysis. C57BL/6J mice were fed a high-fat diet to induce obesity and were orally administered SOL 50% ethanol extract (50, 100, and 200 mg/kg) for 8 weeks. Among the SOL extracts, the 50% EtOH extract considerably inhibited TG accumulation through the downregulation of PPARγ, C/EBPα, and SREBP-1c in 3T3-L1 adipocytes. In addition, the 50% ethanol extract reduced body weight and adipose tissue weight and improved serum lipid profiles through downregulation of PPARγ, C/EBPα, FABP4, and ACC and upregulation of adiponectin and CPT-1 in obese C57BL/6J mice fed a high-fat diet. These results suggested that the SOL 50% EtOH extract may have an antiobesity effect through the regulation of transcription factors related to adipogenesis, lipogenesis, and lipolysis.