Optimization of subculture and DNA extraction steps within the whole genome sequencing workflow for source tracking of Salmonella enterica and Listeria monocytogenes

This work shows that an incubation time reduced to 4–5 h to prepare a culture for DNA extraction followed by an automated DNA extraction can shorten the hands-on time, the turnaround time by 30% and increase the throughput while maintaining the WGS quality assessed by high quality Single Nucleotide...

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Bibliographic Details
Published inJournal of microbiological methods Vol. 151; pp. 66 - 68
Main Authors Gimonet, Johan, Portmann, Anne-Catherine, Fournier, Coralie, Baert, Leen
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.08.2018
Subjects
Automation
DNA extraction
Listeria monocytogenes
Salmonella enterica
Source tracking
Subculture
Listeria monocytogenes
Salmonella enterica
DNA extraction
Automation
Source tracking
Subculture
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Summary:This work shows that an incubation time reduced to 4–5 h to prepare a culture for DNA extraction followed by an automated DNA extraction can shorten the hands-on time, the turnaround time by 30% and increase the throughput while maintaining the WGS quality assessed by high quality Single Nucleotide Polymorphism analysis. •4–5 h incubation of a single colony in BHI is sufficient for DNA extraction.•Automated DNA extraction reduces hands-on time and increases throughput for WGS analysis.•Inoculum can be refrigerated without negatively affecting the time of WGS analysis.
Bibliography:ObjectType-Article-1
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ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2018.06.005