Influence of medium composition, pH and temperature on the growth and viability of Lactobacillus acidophilus

The Lactobacillus acidophilus growth was investigated to find the optimal concentrations of 2 nitrogen sources (thought to be in excess) and 2 organic acids. A Plackett and Burman experimental design was used allowing identification of the more important factors with very few experiments. In the stu...

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Bibliographic Details
Published inBiotechnology letters Vol. 18; no. 7; pp. 775 - 780
Main Authors TAILLANDIER, P, GILIS, F, PORTUGAL, F. R, LAFORCE, P, STREHAIANO, P
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.07.1996
Springer Verlag
Subjects
Biological and medical sciences
Biotechnology
Chemical and Process Engineering
Chemical engineering
Chemical Sciences
Engineering Sciences
Fundamental and applied biological sciences. Psychology
Lactobacillus acidophilus
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Culture medium
Microorganism growth
Experimental design
Microorganism culture
Bacteria
Lactobacillaceae
Environmental factor
Lactobacillus acidophilus
Viability
Optimization
Glucose
Sodium
Nitrogen
Organic Acid
Organic Chemistry
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Summary:The Lactobacillus acidophilus growth was investigated to find the optimal concentrations of 2 nitrogen sources (thought to be in excess) and 2 organic acids. A Plackett and Burman experimental design was used allowing identification of the more important factors with very few experiments. In the studied range, all the factors had a linear effect and a great influence on the final viability. 20 g/L of each nitrogen sources, 3 g sodium-citrate/L and 5 g sodium acetate/L are necessary. pH, temperature and glucose had poor influence.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00127887