Effect of the melatonin nuclear receptor RORα on monochromatic light-induced T-lymphocyte proliferation in chicken thymus

• Published in: Immunology letters Vol. 213; pp. 21 - 29
• Main Authors: Xiong, Juanjuan, Wang, Zixu, Cao, Jing, Dong, Yulan, Chen, Yaoxing
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2019
• Subjects: Animals; Avian Proteins - immunology; Cell Proliferation; Chick; Chickens - immunology; Light; Monochromatic light; NF-κB; Orphan Nuclear Receptors - immunology; Receptors, Melatonin - immunology; RORα; Signal Transduction - immunology; T-lymphocyte proliferation; T-Lymphocytes - immunology; NF-κB; T-lymphocyte proliferation; Monochromatic light; RORα; Chick
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/j.imlet.2019.07.003

[Display omitted] •RL promotes melatonin nuclear receptor RORα expression in chick thymus relative to GL.•RORα plays a negative role in the process of GL-induced T-lymphocyte proliferation.•RORα promotes NF-κB nuclear translocation through upregulating IκB phosphorylation. Present study clarified role of melatonin nuclear receptor RORα in monochromatic light-induced T-lymphocyte proliferation in chicks. Green light elevated plasma melatonin level and organ index, T-lymphocyte proliferation and IL-2 production in thymus, but decreased RORα, p-P65 and p-IκB expressions relative to red light. By contrast, pinealectomy decreased the melatonin content and reversed the stimulatory effect of green light, and resulted in that these thymus parameters were not significantly different among the light-treated groups. Exogenous melatonin supplementation enhanced T-lymphocyte proliferation and IL-2 production in cultured thymocytes. This stimulatory effect of melatonin was reversed by RORα agonist but was enhanced by RORα antagonist. In contrast to RORα antagonist, RORα agonist decreased cytoplasmic P65 level and increased nuclear P65 level. Supplementation with P65 antagonist increased T-lymphocyte proliferation. We conclude that RORα could negatively regulate green light-enhanced T-lymphocyte proliferation in chick thymus by upregulating IκB phosphorylation, which promotes P65 nuclear translocation and NF-κB activation.