Tissue plasminogen activator (t-PA) and plasminogen activator inhibitor type 1 (PAI-1) in diabetic foot syndrome

• Published in: Advances in medical sciences Vol. 62; no. 1; pp. 87 - 91
• Main Authors: Kulwas, Arleta, Lisewska, Beata, Jundziłł, Wiesław, Ruszkowska, Barbara, Drewniak, Wanda, Ruprecht, Zofia, Gadomska, Grażyna, Rość, Danuta
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2017
• Subjects: Adult; Aged; Aged, 80 and over; Biomarkers - blood; Case-Control Studies; Diabetes Mellitus, Type 2 - complications; Diabetic Foot - blood; Diabetic Foot - diagnosis; Diabetic Foot - etiology; Diabetic foot syndrome; Female; Follow-Up Studies; Humans; Male; Middle Aged; PAI-1; Plasminogen Activator Inhibitor 1 - blood; Prognosis; Tissue Plasminogen Activator - blood; tPA; tPA; Diabetic foot syndrome; PAI-1
• ISSN: 1896-1126; 1898-4002
• DOI: 10.1016/j.advms.2016.07.007

The aim was to evaluate tissue plasminogen activator (tPA) and plasminogen activator inhibitor type 1 (PAI-1) concentration using enzyme linked immunosorbent assay method (ELISA) in diabetic foot syndrome (DFS) as compared to a group of healthy people and patients with diabetes mellitus without symptomatic vascular complications (DM2T). Venous blood samples were collected from 90 patients with type 2 diabetes mellitus (30 – DM2T; 60 – DFS). Age-matched controls were also included (n=30). tPA and PAI-1 plasma levels were measured by enzyme-linked immunosorbent assay (ELISA). We found a significantly lower concentration of tPA:Ag in patients with DFS in comparison to the DM2T group; tPA concentrations were significantly higher in DM2T as compared to the control group. We observed significantly lower concentration of PAI-1:Ag in DF patients treated for hypertension as compared to patients without hypertension. The tPA:Ag and PAI-1:Ag concentration analysis in DFS depending on age, gender and BMI did not show any significant differences. A lower concentration of tPA in patients with DFS may be associated with damage to the endothelial cells, especially in the microvasculature, and the sympathetic nervous system.