Complete genome sequence of a novel gammapartitivirus from Penicillium oxalicum RCEF7482

• Published in: Archives of virology Vol. 169; no. 3; p. 48
• Main Authors: Sun, Jing, Wang, Ping, Lu, Hanwen, Yang, Guogen, Huang, Bo
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.03.2024; Springer Nature B.V
• Subjects: amino acids; Annotated Sequence Record; Biological control; Biomedical and Life Sciences; Biomedicine; China; Comparative analysis; Double-stranded RNA; family; Fungal Viruses; Fungi; Gammapartitivirus; genome; Genome, Viral; Genomes; genus; Infectious Diseases; Medical Microbiology; mycoviruses; New species; Nucleotide sequence; nucleotide sequences; Open Reading Frames; Penicillium; Penicillium aurantiogriseum; Penicillium oxalicum; Phylogeny; RNA; RNA Viruses; RNA, Double-Stranded - genetics; RNA, Viral - genetics; RNA-Dependent RNA Polymerase - genetics; Virology; China
• ISSN: 0304-8608; 1432-8798; 1432-8798
• DOI: 10.1007/s00705-024-05987-8

Penicillium oxalicum , an important biocontrol fungus in China, has been a subject of extensive study due to its role in combating various pathogenic fungi. Despite the prevalence of mycoviruses with double-stranded (ds) RNA genomes in filamentous fungi, there has been no screening of mycoviruses in P. oxalicum . In this report, we describe the identification and characterization of a novel dsRNA virus isolated from P. oxalicum , designated as "Penicillium oxalicum partitivirus 1" (PoPV1). The genome of PoPV1 consists of two dsRNA segments, dsRNA1 (1,770 bp) and dsRNA2 (1,584 bp), each containing a single open reading frame (ORF): ORF1 and ORF2. Comparative analysis revealed that the RdRp and CP amino acid sequences of PoPV1 share the highest identity (89.18% and 73.97%, respectively) with those of Penicillium aurantiogriseum partitivirus 1 (PaPV1). Motif analysis based on RdRp amino acid sequences places PoPV1 in the genus Gammapartitivirus within the family Partitiviridae , with a distinctive motif VI (R/KV/ILGDD). Phylogenetic analysis further established a close relationship of PoPV1 to PaPV1, forming a unique clade among the gammapartitiviruses. Consequently, we propose that Penicillium oxalicum partitivirus 1 represents a new species in the genus Gammapartitivirus . This is the first report of a dsRNA virus in P. oxalicum .