Potassium inwardly-rectifying channel subfamily J member 11 (KCNJ11) gene polymorphism in Egyptian type 2 diabetic patients: a single-center study

• Published in: Molecular biology reports Vol. 51; no. 1; p. 1129
• Main Authors: Abdelazem, Abdallah S., Gaber, Osama Abdelaziz, Hussein, Samia, Nasr, Fatma Mahmoud Elsaid, M. Elshorbagy, Eman A., Ibrahim, Sara Mohammed, Abdel-hameed, Abdullah Mohammad, Rashad, Mai Hamdy, El-Shal, Amal S., Abdelnabi, Al-Shabrawy M.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2024; Springer Nature B.V
• Subjects: Adult; Alleles; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Blood glucose; Blood Glucose - metabolism; Body mass index; Case-Control Studies; Cholesterol; clinical examination; Diabetes; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - genetics; Egypt; family; Fasting; Female; Gene frequency; Gene Frequency - genetics; Gene polymorphism; Genetic diversity; Genetic Predisposition to Disease; Genotype; Glycated Hemoglobin - metabolism; glycohemoglobin; Hemoglobin; High density lipoprotein; Histology; Humans; insulin; KCNJ11 gene; Life Sciences; lipid composition; Low density lipoprotein; Male; Middle Aged; Morphology; noninsulin-dependent diabetes mellitus; Original Article; Polymerase chain reaction; Polymorphism; Polymorphism, Single Nucleotide - genetics; Potassium; potassium channels; Potassium Channels, Inwardly Rectifying - genetics; regression analysis; risk; secretion; Single-nucleotide polymorphism; Statistical analysis; subfamily; Triglycerides; Egypt; Lipid profile; Diabetes mellitus; Genetic polymorphism
• ISSN: 0301-4851; 1573-4978; 1573-4978
• DOI: 10.1007/s11033-024-10035-4

Background The KCNJ11 gene belongs to the potassium channel gene family. It has a major role in the secretion of insulin. Genetic variations in KCNJ11 are possibly responsible for the progression of type 2 diabetes mellitus (T2DM). In this study, we investigated the possible correlation between KCNJ11 (rs5210) gene polymorphism and T2DM. Subjects and method This study included 92 individuals divided into two groups. Group 1 included 46 type 2 diabetic patients. Group 2 (control group) included 46 healthy participants. A complete history was taken and a full physical examination was performed. Anthropometric data were measured. Laboratory investigations included fasting blood glucose (FBG), two hours post-prandial blood glucose (2HPPBG), glycated hemoglobin (HbA1c), and fasting lipid profile. KCNJ11 (rs5210) single nucleotide polymorphism was detected by polymerase chain reaction restriction-fragment length polymorphism (PCR–RFLP). Results Both AG and GG genotypes were associated with increased risk for T2DM (OR 5.2, 95% CI 1.32–20.5, P = 0.01 for AG ; and OR 18.2, 95% CI 2.99–31.7, P = 0.002 for GG). Also, the frequency of the G allele was significantly higher in type 2 diabetic patients compared to healthy controls (50% versus 23.9%, respectively). The G allele of rs5210 in KCNJ11 contributed to an increased risk of T2DM (OR 3.18, 95% CI 1.31–7.75, P = 0.01). There was a statistically significant association between increased 2HPPBG and HbA1c levels and the carrier of AG and GG genotypes (P = 0.01 and 0.007, respectively). There was a statistically significant association between total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-c), and high-density lipoprotein-cholesterol (HDL-c) levels and the carrier of AG and GG genotypes (P < 0.001, 0.02, and 0.007, respectively). Regression analysis detected that body mass index (BMI), 2HPPBG, TC, triglycerides (TG), and the G allele of rs5210 in KCNJ11 gene showed a significant association with T2DM (P = 0.004, 0.042, 0.003, 0.006, and 0.01, respectively) while no association was observed with FBG, HbA1c, LDL-c or HDL-c (P = 0.099, 0.123, 0.522, and 0.765, respectively). Conclusion KCNJ11 rs5210 genetic polymorphism may raise the risk for the occurrence of T2DM among Egyptians.