An analysis of growth regulator interactions and gene expression during auxin-induced cell elongation using cloned complementary DNAs to auxin-responsive messenger RNAs

• Published in: Plant physiology (Bethesda) Vol. 77; no. 4; pp. 847 - 850
• Main Authors: Walker, J.C, Legocka, J, Edelman, L, Key, J.L
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.04.1985
• Subjects: ADN; Agronomy. Soil science and plant productions; Analytical, structural and metabolic biochemistry; ARN; AUXINAS; AUXINE; AUXINS; Biological and medical sciences; Cell growth; CELLS; CELLULE; CELULAS; Complementary DNA; CRECIMIENTO; CROISSANCE; Cytokinins; DNA; Economic plant physiology; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; GLYCINE MAX; GROWTH; Growth and development; GROWTH REGULATORS; Hormones; Hypocotyls; INTERACTIONS; Messenger RNA; Other biological molecules; Plant growth substances; RNA; Soybeans; Auxin; Induction; Interaction; Ethylene; Gene expression; Cytokinin; Glycine max; Hypocotyl; Messenger RNA; Leguminosae; Complementary DNA; Dicotyledones; Angiospermae; Plant growth substance; Spermatophyta; Elongation
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.77.4.847

We have examined the effects of cytokinin, fusicoccin, and ethylene on auxin-induced changes in gene expression during auxin-promoted cell elongation in soybean (Glycine max L. Merr. cv Wayne) using cloned cDNAs to two auxin-responsive mRNAs (Walker, Key 1982 Proc Natl Acad Sci USA 79: 7185-7989). RNA blot analyses demonstrate that under conditions of cytokinin inhibition of auxin-promoted cell elongation the levels of these two auxin-responsive mRNAs is unaltered. Fusicoccin-promoted elongation is not associated with an enhanced expression of these two mRNAs, suggesting that the increased levels of these mRNAs observed during auxin-promoted cell elongation are not simply due to enhanced rates of cell elongation. We have also determined that ethylene plays no apparent role in the regulation of expression of these mRNAs. However, the auxins indole-3-acetic acid, 2,4-dichlorophenoxyacetic acid, and α-naphthalene acetic acid all enhance an accumulation of these mRNAs. We conclude that the regulation of these mRNAs is directly dependent on auxin. That auxin-promoted cell elongation is dependent upon the increased accumulation of these mRNAs remains to be determined.