Comparative transcriptomic analysis reveals molecular processes involved in pileus morphogenesis in Pleurotus eryngii under different light conditions

• Published in: Genomics (San Diego, Calif.) Vol. 112; no. 2; pp. 1707 - 1715
• Main Authors: Du, Fang, Zou, Yajie, Hu, Qingxiu, Zhang, Huiying, Ye, Dou
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2020
• Subjects: Gene Expression Regulation, Fungal; Light; Light quality; Light sensing; Melanins - biosynthesis; Morphogenesis; Pileus morphogenesis; Pleurotus - genetics; Pleurotus - growth & development; Pleurotus - metabolism; Pleurotus - radiation effects; Pleurotus eryngii; Transcriptome; Transcriptomics; Light quality; Pleurotus eryngii; Pileus morphogenesis; Transcriptomics; Light sensing
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2019.09.014

Light plays an important role in pileus differentiation in Pleurotus eryngii cultivation, and pileus morphology is influenced by light quality. To understand the effects of light quality on pileus morphology at the transcriptional level, we performed a comparative transcriptomic analysis of pilei grown under blue and red light irradiation. We identified 3959 differentially expressed genes (DEGs) between the blue and red light–treated pilei, which included 1664 up-regulated and 2295 down-regulated genes. These DEGs were significantly associated with light sensing, signal transduction, cell wall degradation and melanogenesis, suggesting that these processes are involved in pileus morphogenesis. Multiple DEGs related to respiratory functions were differentially expressed, suggesting that respiratory activity increased during pileus development regardless of light quality. These results provide a valuable view of the transcriptional changes and molecular processes involved in pileus morphogenesis under different light conditions and provide a foundation for yield improvement and quality control of P. eryngii. •The variations underlying pileus morphogenesis under different light qualities were revealed at the transcriptional level.•Genes involved in sensing and transducing blue- and red-light signals were correspondingly up-regulated, respectively.•The expression level of tyrosinase related to pigment formation was significantly up-regulated under blue light condition.