Purification and establishment of ELISA for vitellogenin of Japanese sardine ( Sardinops melanostictus)

Two female-specific serum proteins (FSSPs) were detected immunologically in estradiol-treated Japanese sardine Sardinops melanostictus. The major FSSP was demonstrated to be a high molecular estradiol-inducible glycolipophosphoprotein with an immunological relation to a major yolk protein, and was s...

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Published inComparative biochemistry and physiology. B, Comparative biochemistry Vol. 109; no. 4; pp. 545 - 555
Main Authors Matsubara, Takahiro, Wada, Tokio, Hara, Akihiko
Format Journal Article
LanguageEnglish
Published Elsevier Inc 1994
Subjects
antiserum
ELISA
enzyme-linked immunosorbent assay
estradiol
Female
FSSP
induction
Marine
Purification
Reproduction
Sardine
Sardinops
Sardinops melanosticta
Teleost
Vitellogenesis
Vitellogenin
vitellogenins
Japan
Sardine
Teleost
Reproduction
Purification
Vitellogenesis
Female
FSSP
Vitellogenin
ELISA
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Summary:Two female-specific serum proteins (FSSPs) were detected immunologically in estradiol-treated Japanese sardine Sardinops melanostictus. The major FSSP was demonstrated to be a high molecular estradiol-inducible glycolipophosphoprotein with an immunological relation to a major yolk protein, and was suggested to be vitellogenin (VTG). VTG was purified using negative immunoaffinity chromatography. The isolated VTG was used for raising the specific antiserum against VTG. A homologous enzyme-linked immunosorbent assay (ELISA) was developed using the antiserum and the isolated VTG. The sensitivity range of the ELISA was 44 ng/ml to 2670 ng/ml of VTG concentration for the conditions used in our investigation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0305-0491
DOI:10.1016/0305-0491(94)90116-3