Purification and establishment of ELISA for vitellogenin of Japanese sardine ( Sardinops melanostictus)
Two female-specific serum proteins (FSSPs) were detected immunologically in estradiol-treated Japanese sardine Sardinops melanostictus. The major FSSP was demonstrated to be a high molecular estradiol-inducible glycolipophosphoprotein with an immunological relation to a major yolk protein, and was s...
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Published in | Comparative biochemistry and physiology. B, Comparative biochemistry Vol. 109; no. 4; pp. 545 - 555 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Inc
1994
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Subjects | |
Online Access | Get full text |
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Summary: | Two female-specific serum proteins (FSSPs) were detected immunologically in estradiol-treated Japanese sardine
Sardinops melanostictus. The major FSSP was demonstrated to be a high molecular estradiol-inducible glycolipophosphoprotein with an immunological relation to a major yolk protein, and was suggested to be vitellogenin (VTG). VTG was purified using negative immunoaffinity chromatography. The isolated VTG was used for raising the specific antiserum against VTG. A homologous enzyme-linked immunosorbent assay (ELISA) was developed using the antiserum and the isolated VTG. The sensitivity range of the ELISA was 44 ng/ml to 2670 ng/ml of VTG concentration for the conditions used in our investigation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0305-0491 |
DOI: | 10.1016/0305-0491(94)90116-3 |