Evaluation of profile and functionality of memory T cells in pulmonary tuberculosis

• Published in: Immunology letters Vol. 192; pp. 52 - 60
• Main Authors: Tonaco, Marcela M., Moreira, Jôsimar D., Nunes, Fernanda F.C., Loures, Cristina M.G., Souza, Larissa R., Martins, Janaina M., Silva, Henrique R., Porto, Arthur Henrique R., Toledo, Vicente Paulo C.P., Miranda, Silvana S., Guimarães, Tânia Mara P.D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2017
• Subjects: Adult; Asymptomatic Diseases; CD27; CD4-Positive T-Lymphocytes - immunology; CD45RO; CD8-Positive T-Lymphocytes - immunology; Cells, Cultured; Female; Humans; IFN-ɣ; IL-2; IL17; Immunologic Memory; Immunophenotyping; Interferon-gamma - metabolism; Interleukin-17 - metabolism; Lung - immunology; Lung - microbiology; Lymphocytes; Male; Memory cells; Middle Aged; Mycobacterium tuberculosis; Mycobacterium tuberculosis - immunology; Surface markers; TNF-α; Tuberculosis; Tuberculosis, Pulmonary - immunology; Tumor Necrosis Factor-alpha - metabolism; Memory cells; IFN-ɣ; IL-2; TMC; IL17; LTBI; PBMC; TME; TB; IFN- ɣ; Tuberculosis; TNF-α; Mycobacterium tuberculosis; CD45RO; TBPT; Lymphocytes; IL-17; Mtb-Ags; CD27; Mtb; Surface markers; ATBP
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/j.imlet.2017.10.014

•Patients with active pulmonary tuberculosis − highest proportion of cells T CD4+ TMC IFN-ɣ+ e TNF-α+.•LT CD4+TMC would be an important target of study for the development of more effective vaccines.•Patients after treatment of pulmonary TB − highest proportion of cells T CD4+ TMC IL-17+.•Individuals with latent infection by M. tuberculosis and treated for pulmonary tuberculosis − greater response of T lymphocytes CD8+ effector IFN-ɣ+.•Stimulation of 144h with high antigenic load and elimination of the pathogen, more significant generation of T central memory cells. The cells T CD4+ T and CD8+ can be subdivided into phenotypes naïve, T of central memory, T of effector memory and effector, according to the expression of surface molecules CD45RO and CD27. The T lymphocytes are cells of long life with capacity of rapid expansion and function, after a new antigenic exposure. In tuberculosis, it was found that specific memory T cells are present, however, gaps remain about the role of such cells in the disease immunology. In this study, the phenotypic profile was analyzed and characterized the functionality of CD4+ T lymphocytes and CD8+ T cells of memory and effector, in response to specific stimuli in vitro, in patients with active pulmonary TB, compared to individuals with latent infection with Mycobacterium tuberculosis the ones treated with pulmonary TB. It was observed that the group of patients with active pulmonary tuberculosis was the one which presented the highest proportion of cells T CD4+ of central memory IFN-ɣ+ e TNF-α+, suggesting that in TB, these T of central memory cells would have a profile of protective response, being an important target of study for the development of more effective vaccines; this group also developed lower proportion of CD8+ T effector lymphocytes than the others, a probable cause of specific and less effective response against the bacillus in these individuals; the ones treated for pulmonary tuberculosis were those who developed higher proportion of T CD4+ of memory central IL-17+ cells, indicating that the stimulation of long duration, with high antigenic load, followed by elimination of the pathogen, contribute to more significant generation of such cells; individuals with latent infection by M. tuberculosis and treated for pulmonary tuberculosis, showed greater response of CD8+ T effector lymphocytes IFN-ɣ+ than the controls, suggesting that these cells, as well as CD4+ T lymphocytes, have crucial role of protection against M. tuberculosis. These findings have contributed to a better understanding of the immunologic changes in M. tuberculosis infection and the development of new strategies for diagnosis and prevention of tuberculosis.