CRISPR Lights up In Situ Protein Evolution

In this issue of Cell Chemical Biology, Erdogan et al. (2020) describe a new CRISPR/Cas9-based strategy for performing directed evolution of mammalian proteins in situ. Using this technique to select functional mRuby3 variants within lysosomes, they identify mCRISPRed, a fluorescent protein that dis...

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Bibliographic Details
Published inCell chemical biology Vol. 27; no. 5; pp. 475 - 478
Main Authors Kerek, Evan M, Cromwell, Christopher R, Hubbard, Basil P
Format Journal Article
LanguageEnglish
Published United States 21.05.2020
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Summary:In this issue of Cell Chemical Biology, Erdogan et al. (2020) describe a new CRISPR/Cas9-based strategy for performing directed evolution of mammalian proteins in situ. Using this technique to select functional mRuby3 variants within lysosomes, they identify mCRISPRed, a fluorescent protein that displays robust stability and activity at low pH.
ISSN:2451-9456
2451-9448
2451-9456
DOI:10.1016/j.chembiol.2020.05.005