Bromobenzene-induced hepatotoxicity in male rats: the protective effect of flaxseed

• Published in: Toxicological and environmental chemistry Vol. 94; no. 5; pp. 1000 - 1013
• Main Authors: El-Sharaky, A.S, Newairy, A.A, Eweda, S.M, Kamel, M.A
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis Group 01.05.2012; Taylor & Francis Ltd
• Subjects: antioxidant activity; antioxidant enzymes; Antioxidants; apoptosis; bromobenzene; caspase-3; cytochrome P-450; Cytotoxicity; DNA fragmentation; drug metabolizing enzymes; drugs; Enzymes; flaxseeds; glutathione peroxidase; glutathione-disulfide reductase; hepatotoxicity; linseed; lipid peroxidation; liver; Metabolites; nitric oxide; nitric oxide products; oral administration; Oxidative stress; pretreatment; protective effect; rats; Seeds; superoxide dismutase
• ISSN: 1029-0486; 0277-2248; 1029-0486
• DOI: 10.1080/02772248.2012.675667

The metabolites of bromobenzene (BB) are hepatotoxic. The aim of this study was to determine the efficacy of different doses of flaxseed extract in alleviating BB-hepatotoxicity in male albino rats. Oxidative stress parameters, drug metabolizing enzymes, a pro-inflammatory marker, an apoptotic marker, and DNA fragmentation pattern were also assessed. Animals were divided into five groups treated by intragastric intubation as follows: control, BB-treated 460 mg kg⁻¹ BW alone; three animal groups (III, IV, V) were treated concurrently with 460 mg kg⁻¹ BB daily for 3 weeks and different doses of flaxseeds extract: 100, 200, or 300 mg kg⁻¹ BW. Oral treatment of BB produced a significant decrease in activities of antioxidant enzymes superoxide dismutase and glutathione peroxidase and glutathione levels, while activities of glutathione reductase and drug-metabolizing enzymes; glutathione-S-transferases and cytochrome P450 were enhanced. BB-treatment resulted in enhanced production of nitric oxide and activation of COX-2 and caspase-3. Pre-treatment with different doses of flaxseeds extract prior and during BB-treatment protected liver against BB-induced hepatotoxicity. The lower dose of flaxseed extract (100 mg kg⁻¹) was most effective one.