448-Detector optical recording system: development and application to Aplysia gill-withdrawal reflex

The spatial resolution of a fast, multichannel, optical recording apparatus has been improved in an attempt to increase the completeness of optical recordings of neuron activity responsible for the Aplysia gill-withdrawal reflex. A new optical apparatus was developed, increasing the number of pixels...

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Bibliographic Details
Published inIEEE transactions on biomedical engineering Vol. 39; no. 1; pp. 26 - 36
Main Authors Nakashima, M., Yamada, S., Shiono, S., Maeda, M., Satoh, F.
Format Journal Article
LanguageEnglish
Published New York, NY IEEE 01.01.1992
Institute of Electrical and Electronics Engineers
Subjects
Abdomen
Action Potentials
Animals
Aplysia
Biological and medical sciences
Biomedical optical imaging
Central nervous system
Electrophysiology
Evaluation Studies as Topic
Fundamental and applied biological sciences. Psychology
Laboratories
Microelectrodes
Microscopy, Fluorescence - methods
Microscopy, Fluorescence - standards
Neural networks
Neurons
Optical arrays
Optical detectors
Optical recording
Optical sensors
Reflex
Signal Processing, Computer-Assisted
Vertebrates: nervous system and sense organs
Gill withdrawal reflex
Aplysia californica
Electrophysiology
Instrumentation
Action potential
Nervous system
Gastropoda
Neural network
Vertebrata
Detector
Neuron
Optical recording
Animal
Pisces
Invertebrata
Mollusca
Spatial resolution
Simultaneous recording
Biomedical engineering
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Summary:The spatial resolution of a fast, multichannel, optical recording apparatus has been improved in an attempt to increase the completeness of optical recordings of neuron activity responsible for the Aplysia gill-withdrawal reflex. A new optical apparatus was developed, increasing the number of pixels to 448. Action potential activity from 168 to 192 neurons could be detected. Hence, the number of optically detected neurons increased about 2.5 times in comparison with a previously used 100- or 124-detector optical apparatus. Because optically detected action potential signals were shown to be due to the cell body of a neuron, it was possible to know its position as well as its approximate size. The distribution of the detected active neurons and those neurons that showed an apparent change in spike frequency to the siphon stimulation were determined.< >
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ISSN:0018-9294
1558-2531
DOI:10.1109/10.108124