Proteomic Analysis of Developing Somatic Embryos of Coffea arabica

• Published in: Plant molecular biology reporter Vol. 30; no. 6; pp. 1393 - 1399
• Main Authors: Tonietto, Ângela, Sato, Juliana Hiromi, Teixeira, João Batista, de Souza, Emanuel M., Pedrosa, Fabio O., Franco, Octávio Luiz, Mehta, Angela
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.12.2012; Springer Nature B.V
• Subjects: Bioinformatics; Biomedical and Life Sciences; Coffea arabica; Coffee; Developmental stages; Embryonic growth stage; Embryos; Globulins; Life Sciences; Liquid nitrogen; Mass spectrometry; Mass spectroscopy; Metabolomics; Original Paper; pH effects; Phenols; Phosphopyruvate hydratase; Plant Breeding/Biotechnology; Plant Sciences; Proteins; Proteomics; Somatic embryogenesis; Molecular markers; 2-DE; Protein profile; Mass spectrometry; Somatic embryogenesis
• ISSN: 0735-9640; 1572-9818
• DOI: 10.1007/s11105-012-0425-7

Differential protein profiles of three stages of somatic embryogenesis, including globular, torpedo, and cotyledonary somatic embryos, of Coffea arabica cv. Catuaí Vermelho were analyzed in an attempt to better understand somatic embryogenesis in coffee plants. Somatic embryos at these different stages of development were collected from in vitro-grown cultures, and then macerated in liquid nitrogen. Proteins were extracted with phenol and further quantified using the Bradford method. The bidimensional electrophoresis analysis revealed a wide range of proteins ranging between 10 and 160 kDa and of pH values ranging from 3 to 10. Several differentially expressed proteins were identified by mass spectrometry, and some were found to be specific to these different stages of somatic embryogenesis in coffee. The enolase and 11S storage globulin proteins, for example, could be used as molecular markers for somatic embryo development stages and for embryogenic and non-embryogenic genotype differentiation, respectively.