A micro-Raman spectroscopy study of inflammatory condition of human cervix: Probing of tissues and blood plasma samples

• Published in: Photodiagnosis and photodynamic therapy Vol. 39; p. 102948
• Main Authors: Barik, Ajaya Kumar, M, Sanoop Pavithran, N, Mithun, Pai, Muralidhar V, Upadhya, Rekha, Pai, Abhilash K, Lukose, Jijo, Chidangil, Santhosh
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2022
• Subjects: Blood plasma; Cervical cancer; Cervical tissue; Cervicitis; Liquid biopsy; Micro-Raman spectroscopy; Support vector machine; Liquid biopsy; Cervicitis; Cervical tissue; Support vector machine; Cervical cancer; Micro-Raman spectroscopy; Blood plasma; cervicitis; Support Vector Machine; blood plasma; cervical tissue; cervical cancer
• ISSN: 1572-1000; 1873-1597
• DOI: 10.1016/j.pdpdt.2022.102948

•There is a high demand for a cost effective & minimally invasive technique for cervical cancer/ cervicitis discrimination.•Raman spectroscopy can be a potential screening tool for probing cervical tissue abnormalities from blood plasma.•Biochemical alterations in cervical tissues from Raman spectra have been well correlated with the variations in plasma.•Support vector machine model discriminates malignant & non-malignant samples with sensitivity and specificity above 84%. The present study explores the application of the micro-Raman spectroscopy technique to discriminate normal and cervicitis condition from cervical malignancy by analyzing the Raman signatures of tissues and plasma samples of the same subjects. The Raman peaks from tissue samples at 1026 cm−1,1298 cm−1 and 1243 cm−1 are attributed to glycogen, fatty acids and collagen and are found to be reliable signatures capable of identifying cervicitis and normal condition from cervical cancer. The Raman signatures from plasma samples belonging to carbohydrates (578 cm−1), lipids (1059 cm−1) and nucleic acids (1077 cm−1,1341 cm−1 and 1357 cm−1) are quite useful to classify various pathological conditions of cervix at par with tissue based diagnosis. The PCA-SVM based classification of the spectral data indicates the potential of Raman spectroscopy based liquid biopsy to rule out false diagnosis of cervicitis as cervical malignancy.