5-Aminolaevulinic acid photodynamic therapy suppresses lipid secretion of primary sebocytes through AMPK/SREBP-1 pathway

• Published in: Photodiagnosis and photodynamic therapy Vol. 36; p. 102537
• Main Authors: Yang, Jiayi, Shi, Lei, Xu, Detian, Liu, Jia, Zhang, Linglin, Liu, Xiaojing, Zeng, Qingyu, Wang, Xiuli
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2021
• Subjects: Acne; Acne Vulgaris - drug therapy; ALA-PDT; Aminolevulinic Acid - pharmacology; Aminolevulinic Acid - therapeutic use; AMP-Activated Protein Kinases; Animals; Cricetinae; Human sebocytes; Humans; lipid secretion; Lipids - therapeutic use; Photochemotherapy - methods; Photodynamic Therapy; Photosensitizing Agents - pharmacology; Photosensitizing Agents - therapeutic use; SREBP-1; Sterol Regulatory Element Binding Protein 1; lipid secretion; Photodynamic Therapy; Human sebocytes; SREBP-1; Acne; ALA-PDT
• ISSN: 1572-1000; 1873-1597
• DOI: 10.1016/j.pdpdt.2021.102537

•ALA-PDT inhibits lipid secretion of primary human sebocytes and induces atrophy of sebaceous gland.•ALA-PDT suppresses the expression of SREBP-1 in sebocytes.•ALA-PDT suppresses sebum secretion in the treatment of acne vulgaris through down-regulating the AMPK/SREBP-1 pathway, providing a potential mechanism of ALA-PDT for acne vulgaris. Acne vulgaris is a chronic inflammatory skin disease around pilosebaceous unit. 5-Aminolaevulinic acid photodynamic therapy (ALA-PDT) is an effective therapy for severe acne vulgaris. However, its specific treatment mechanism remains unclear. In the present study, we investigated the potential mechanism of how ALA-PDT induced lipid secretion inhibition in acne vulgaris. Primary human sebocytes and sebaceous gland of golden hamster were treated with/without ALA-PDT. Cell viability was evaluated by Live/Dead Cell assay. Fluorescence microscope was used to observe lipids secretion in sebocytes after Nile red staining. The expression of SREBP-1 after ALA-PDT was evaluated by qRT-PCR. Regulation of ALA-PDT on AMPK/SREBP-1 was evaluated by western blot. The results showed that ALA-PDT suppressed lipid secretion of primary human sebocytes. In addition, ALA-PDT could inhibit the expression of SREBP-1 in vitro. We also found that ALA-PDT activated AMPK pathway, down-regulating the expression of SREBP-1 in sebocytes after ALA-PDT. These findings elucidate that ALA-PDT suppresses lipid secretion through AMPK/SREBP-1 pathway in treatment of acne vulgaris.