Pathogenicity of avian leukosis viruses

Three methods were used in attempts to obtain non-oncogenic avian leukosis virus for possible use as an immunoprophylactic agent for the control of lymphoid leukosis in chickens. These were: 1) isolate a nononcogenic virus from commercial breeder flocks experiencing very little or no lymphoid leukos...

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Bibliographic Details
Published inAvian diseases Vol. 26; no. 3; p. 553
Main Authors Okazaki, W, Purchase, H G, Crittenden, L B
Format Journal Article
LanguageEnglish
Published United States 01.07.1982
Subjects
Animals
Avian Leukosis - microbiology
Avian Leukosis Virus - genetics
Avian Leukosis Virus - growth & development
Avian Leukosis Virus - pathogenicity
Chick Embryo
Chickens
Fibroblasts
Quail
Recombination, Genetic
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Summary:Three methods were used in attempts to obtain non-oncogenic avian leukosis virus for possible use as an immunoprophylactic agent for the control of lymphoid leukosis in chickens. These were: 1) isolate a nononcogenic virus from commercial breeder flocks experiencing very little or no lymphoid leukosis; 2) obtain a non-oncogenic recombinant from mixed infection of a strain with low oncogenicity, Rous-associated virus-60 (RAV-60), with RAV-1 or RAV-2 in cell culture; and 3) attempt to attenuate subgroup A avian leukosis virus by serial passage in avian cell culture. Of 43 isolates obtained from field sources, all were pathogenic except one, and its pathogenicity was questionable because of the low amount of virus tested. All 42 clones from mixed infection of highly oncogenic and poorly oncogenic virus and all clones passaged serially in cell culture were oncogenic.
Bibliography:L
L73
ISSN:0005-2086
1938-4351
DOI:10.2307/1589901