Coexpression of thyroid hormone receptor isoforms in mouse oligodendrocytes
Double and triple immunocytochemistry with stage‐specific markers and specific antireceptor antibodies was used to study expression of nuclear thyroid hormone receptor (TR) isoforms in cultured mouse oligodendrocytes. To evaluate the coexpression of each TR isoform, antibodies were raised in rabbits...
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Published in | Journal of neuroscience research Vol. 67; no. 1; pp. 106 - 113 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
John Wiley & Sons, Inc
01.01.2002
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Subjects | |
Online Access | Get full text |
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Summary: | Double and triple immunocytochemistry with stage‐specific markers and specific antireceptor antibodies was used to study expression of nuclear thyroid hormone receptor (TR) isoforms in cultured mouse oligodendrocytes. To evaluate the coexpression of each TR isoform, antibodies were raised in rabbits and mice against specific regions of α1‐TR and α2‐TR common to both α isoforms and β1‐TR. Their specificities were assessed by Western blotting and by immunocytochemistry on rat hepatocytes. Oligodendrocyte subpopulations were found to coexpress the α‐ and β1‐TR epitopes at defined developmental stages. Both α‐ and β1‐TR isoforms are colocalized in oligodendrocytes during an early stage identified by the marker OL‐1, before 2′,3′‐cyclic nucleotide 3′‐phosphohydrolase is expressed. Expression of β1‐TR varies during maturation, and that of α‐TR decreases during terminal maturation. © 2002 Wiley‐Liss, Inc. |
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Bibliography: | ArticleID:JNR10111 ark:/67375/WNG-R5HGT4LG-C istex:E47757B870BF43B020FF6BC229354B818B482482 The first two authors contributed equally to this paper. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0360-4012 1097-4547 |
DOI: | 10.1002/jnr.10111 |