Fabrication of Graphene-Quantum Dots Composites for Sensitive Electrogenerated Chemiluminescence Immunosensing
A novel strategy is reported for the fabrication of poly(diallyldimethylammonium chloride) (PDDA)‐protected graphene–CdSe (P‐GR‐CdSe) composites. An advanced electrogenerated chemiluminescence (ECL) immunosensor is proposed for the sensitive detection of human IgG (HIgG) by using the as‐prepared P‐G...
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Published in | Advanced functional materials Vol. 21; no. 5; pp. 869 - 878 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
WILEY-VCH Verlag
08.03.2011
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | A novel strategy is reported for the fabrication of poly(diallyldimethylammonium chloride) (PDDA)‐protected graphene–CdSe (P‐GR‐CdSe) composites. An advanced electrogenerated chemiluminescence (ECL) immunosensor is proposed for the sensitive detection of human IgG (HIgG) by using the as‐prepared P‐GR‐CdSe composites. The P‐GR‐CdSe composite film shows high ECL intensity, good electronic conductivity, fast response, and satisfactory stability, all of which holds great promise for the fabrication of ECL biosensors with improved sensitivity. After two successive steps of amplification via the conjugation of PDDA and gold nanoparticles (GNPs) in the film, high ECL intensity is observed. The ECL immunosensor has an extremely sensitive response to HIgG in a linear range of 0.02–2000 pg mL−1 with a detection limit of 0.005 pg mL−1. The proposed sensor exhibits high specificity, good reproducibility, and long‐term stability, and may become a promising technique for protein detection.
Specific, stable, sensitive: Poly (diallyldimethylammonium chloride) (PDDA)‐protected graphene–CdSe composites were prepared and used for the construction of an advanced electrogenerated‐chemiluminescence immunosensor combined with amplification of PDDA and gold nanoparticles by layer‐by‐layer assembly. The resulting sensor detected the concentration of human IgG with a low detection limit. |
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Bibliography: | National Natural Science Foundation of China for the Key program - No. 20635020 ark:/67375/WNG-FTNCK9HR-T National Basic Research Program of China - No. 2011CB933502 ArticleID:ADFM201001550 Creative Research Group - No. 20821063 General program - No. 50972058 istex:3C13B6DD8D9375683BADDFEC7DAC15383AC7CE0C ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1616-301X 1616-3028 1616-3028 |
DOI: | 10.1002/adfm.201001550 |