Description of Cylindrospermum solincola sp. nov. from Jammu and Kashmir, India and Further Insights into the Ecological Distribution and Morphological Attributes of Cylindrospermum badium

Two cyanobacterial strains KUT1-PS and 18C-PS were collected from the soil surface and vernal pool, respectively, from the Basantgarh village, Udhampur district of the union territory of Jammu and Kashmir, India and characterized by a polyphasic approach. The morphological characterization indicated...

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Published inDiversity (Basel) Vol. 15; no. 5; p. 592
Main Authors Kumar, Naresh, Saraf, Aniket, Pal, Sagarika, Singh, Prashant
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 25.04.2023
Subjects
16S rRNA gene
16S-23S ITS
Algae
Cyanobacteria
Cylindrospermum
Ecological distribution
India
Morphology
New species
Nomenclature
Phylogenetics
Phylogeny
polyphasic approach
Protein structure
Protozoa
rRNA 16S
Secondary structure
Soil surfaces
Structural analysis
Taxonomy
United States > US
Jammu & Kashmir
India
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Summary:Two cyanobacterial strains KUT1-PS and 18C-PS were collected from the soil surface and vernal pool, respectively, from the Basantgarh village, Udhampur district of the union territory of Jammu and Kashmir, India and characterized by a polyphasic approach. The morphological characterization indicated that both the strains showed typical Cylindrospermum-like morphology and probably belonged to the genus Cylindrospermum. Further, phylogenetic interpretations at the genus level were made using the 16S rRNA gene while the 16S-23S ITS region phylogenetic analysis and secondary structure analysis were conducted to enhance the resolution at the species level. The results from the comparative morphological analysis, the 16S rRNA gene percent similarity and phylogenetic analyses, the 16S-23S ITS percent dissimilarity and the ITS secondary structure analyses provided enough evidence that the strain 18C-PS is a representative of Cylindrospermum badium, providing further insights into its ecological distribution and morphological attributes. Additionally, the strain KUT1-PS was a novel species of the genus Cylindrospermum and is referred to herein as Cylindrospermum solincola sp. nov., in accordance with the International Code of Nomenclature for algae, fungi and plants. This study also discusses the importance of comparing the newly sequenced strains with previously established species before making final taxonomic interpretations.
ISSN:1424-2818
1424-2818
DOI:10.3390/d15050592