Complementation studies of the DnaK-DnaJ-GrpE chaperone machineries from Vibrio harveyi and Escherichia coli, both in vivo and in vitro

• Published in: Archives of microbiology Vol. 182; no. 6; pp. 436 - 449
• Main Authors: Zmijewski, Michał A, Kwiatkowska, Joanna M, Lipińska, Barbara
• Format: Journal Article
• Language: English
• Published: Germany Springer Nature B.V 01.12.2004
• Subjects: Adenosine Triphosphatases - metabolism; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - physiology; Cloning, Molecular; DNA, Bacterial - isolation & purification; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - physiology; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Escherichia coli Proteins - physiology; Gene Order; Genetic Complementation Test; Heat-Shock Proteins - genetics; Heat-Shock Proteins - physiology; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins - genetics; HSP70 Heat-Shock Proteins - metabolism; Indicator species; Luciferases - metabolism; Marine environment; Marine pollution; Microbiology; Molecular Chaperones - genetics; Molecular Chaperones - isolation & purification; Molecular Chaperones - physiology; Molecular Sequence Data; Multigene Family; Mutation; Protein Folding; Recombinant Proteins - genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Vibrio; Vibrio - genetics; Vibrio - physiology; Vibrio harveyi
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-004-0727-8

The marine bacterium Vibrio harveyi is a potential indicator organism for evaluating marine environmental pollution. The DnaK-DnaJ-GrpE chaperone machinery of V. harveyi has been studied as a model of response to stress conditions and compared to the Escherichia coli DnaK system. The genes encoding DnaK, DnaJ and GrpE of V. harveyi were cloned into expression vectors and grpE was sequenced. It was found that V. harveyi possesses a unique organization of the hsp gene cluster (grpE-gltP-dnaK-dnaJ), which is present exclusively in marine Vibrio species. In vivo experiments showed that suppression of the E. coli dnaK mutation by V. harveyi DnaK protein was weak or absent, while suppression of the dnaJ and grpE mutations by V. harveyi DnaJ and GrpE proteins was efficient. These results suggest higher species-specificity of the DnaK chaperone than the GrpE and DnaJ cochaperones. Proteins of the DnaK chaperone machinery of V. harveyi were purified to homogeneity and their efficient cooperation with the E. coli chaperones in the luciferase refolding reaction and in stimulation of DnaK ATPase activity was demonstrated. Compared to the E. coli system, the purified DnaK-DnaJ-GrpE system of V. harveyi exhibited about 20% lower chaperoning activity in the luciferase reactivation assay. ATPase activity of V. harveyi DnaK protein was at least twofold higher than that of the E. coli model DnaK but its stimulation by the cochaperones DnaJ and GrpE was significantly (10 times) weaker. These results indicate that, despite their high structural identity (approximately 80%) and similar mechanisms of action, the DnaK chaperones of closely related V. harveyi and E.coli bacteria differ functionally.