Use of N-acetylpsychosine as internal standard for quantitative high-performance liquid chromatographic analysis of glycosphingolipids

The use of N-acetylpsychosine as an internal standard for the quantitative high-performance liquid chromatography (HPLC) of p-nitrobenzoyl derivatives of glycosphingolipids is described. It is suitable because the chromogen reacts on equimolar basis with both N-acetylpsychosine and sample glycosphin...

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Bibliographic Details
Published inJournal of chromatography Vol. 555; no. 1-2; p. 267
Main Authors Cecconi, O, Ruggieri, S, Mugnai, G
Format Journal Article
LanguageEnglish
Published Netherlands 30.08.1991
Subjects
Animals
Chromatography, High Pressure Liquid - methods
Fibrosarcoma - chemistry
Fibrosarcoma - pathology
Glycosphingolipids - analysis
Mice
Psychosine - analogs & derivatives
Reference Standards
Tumor Cells, Cultured - chemistry
Tumor Cells, Cultured - pathology
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Summary:The use of N-acetylpsychosine as an internal standard for the quantitative high-performance liquid chromatography (HPLC) of p-nitrobenzoyl derivatives of glycosphingolipids is described. It is suitable because the chromogen reacts on equimolar basis with both N-acetylpsychosine and sample glycosphingolipids. The use of N-acetylpsychosine as an internal standard was validated by determining the glycosphingolipid content of a system of metastatic variants selected from a murine fibrosarcoma line (T3 cells). Reproducible results were obtained throughout several quantitative analyses of cellular glycosphingolipids and it was possible to determine the glycosphingolipid content of as few as 5 x 10(6) cells.
DOI:10.1016/S0021-9673(01)87188-0