Detection of several harmful algal species by sandwich hybridization integrated with a nuclease protection assay

• Published in: Harmful algae Vol. 8; no. 5; pp. 651 - 657
• Main Authors: Zhen, Yu, Mi, Tiezhu, Yu, Zhigang
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.06.2009; Elsevier
• Subjects: Algae; Animal and plant ecology; Animal, plant and microbial ecology; Autoecology; Biological and medical sciences; Detection; Fundamental and applied biological sciences. Psychology; Harmful algal blooms; Marine; Plant cytology, morphology, systematics, chorology and evolution; Plants and fungi; Sandwich hybridization integrated with a nuclease protection assay; Thallophyta; Detection; Harmful algal blooms; Sandwich hybridization integrated with a nuclease protection assay; Assay; Aquatic environment; nuclease protection assay; Algae; Bloom; Sandwich hybridization integrated with a; Hybridization; Protection; Harmful alga
• ISSN: 1568-9883; 1878-1470
• DOI: 10.1016/j.hal.2008.12.001

The frequent occurrence of harmful algal blooms (HABs) is a pressing topic in marine research. An integrated sandwich hybridization and nuclease protection assay was established to qualitatively and quantitatively detect 12 harmful algal species. This method demonstrated good reliability, specificity and accuracy for analyzing samples from individual and mixed cultures, as well as field collection, and cell volumes were positively correlated to the slopes of calibration curves. The lowest quantitative detection limits were those concentrations observed during blooms; thus, this technique provides an efficient alternative to microscopy for rapid identification and quantitation of harmful algal species and could be routinely used to monitor phytoplankton in field surveys.