Genome-wide identification and expression analysis of the Nitrile-specifier proteins (NSPs) in Brassica napus

• Published in: Current plant biology Vol. 38; p. 100342
• Main Authors: Zhai, Tingting, Teng, Jun, Gu, Mengjie, Wang, Yanjiao, Qu, Ke, Yang, Wei, Zhang, Shuxin
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2024; Elsevier
• Subjects: Brassica napus; Genome-wide; Glucosinolates; Hormones; Nitrile-specifier proteins; Brassica napus; Glucosinolates; Nitrile-specifier proteins; Genome-wide; Hormones
• ISSN: 2214-6628; 2214-6628
• DOI: 10.1016/j.cpb.2024.100342

The complete exploitation of Brassica napus plants is a study hotspot, since it is an essential oil crop that is widely cultivated across the world. Glucosinolate is a unique secondary metabolite in the Brassicaceae family, and its content has a substantial influence on rapeseed development and quality. Its degradation products have various physiological functions, among which Nitrile specific proteins (NSPs) can hydrolyze glucosinolate and hence influence the balance of plant immunity and growth. However, the related gene families in B. napus have not been investigated. Here, 72 NSP family members were discovered and described in B. napus based on their sequence structures, physiological correlations, phonological locations, and expression levels. According to collinearity studies, the NSP proteins in B. napus and A. thaliana are closely related. Analysis of BnNSP expression patterns in various tissues revealed that BnNSPs exhibit high tissue specificity, implying that BnNSPs may play distinguish functions in various developmental phases. We discovered that NSPs may be regulated by hormones such as abscisic acid (ABA), gibberellin (GA), and metallic jasmonate (MeJA) based on the expression of NSPs during hormone treatment. The results provide valuable information for the future functional characterization of BnNSP genes. •A total of 72 BnNSPs genes were first identified in the Brassica napus genome.•Phylogeny, gene structure, motif composition, chromosomal localization, collinearity and variation of BnNSPs were analyzed.•miRNA target and phosphorylation sites prediction reveal some important protein modification and gene regulation information.•BnNSP6 and BnNSP63 showed their putative role in response to hormones.