Metadherin regulates radioresistance in cervical cancer cells

• Published in: Oncology reports Vol. 27; no. 5; pp. 1520 - 1526
• Main Authors: YING ZHAO, MORAN, Meena S, QIFENG YANG, QIAO LIU, CUNZHONG YUAN, SHUHUI HONG, BEIHUA KONG
• Format: Journal Article
• Language: English
• Published: Athens Spandidos 01.05.2012
• Subjects: Apoptosis - genetics; Apoptosis - radiation effects; Biological and medical sciences; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Cell Line, Tumor; DNA Breaks, Double-Stranded - radiation effects; Female; Female genital diseases; G2 Phase Cell Cycle Checkpoints - genetics; G2 Phase Cell Cycle Checkpoints - radiation effects; Gene Silencing; Gynecology. Andrology. Obstetrics; Humans; Medical sciences; Proto-Oncogene Proteins c-bcl-2 - metabolism; Radiation Tolerance - genetics; RNA, Small Interfering - metabolism; Tumors; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - metabolism; Treatment resistance; Radiosensitivity; Malignant tumor; Radiotherapy; DNA double-strand breaks repair; metadherin; Female genital diseases; Double stranded DNA; Cancerology; Treatment; Cell death; Cell cycle; Uterine cervix diseases; Cervical cancer; Tumor cell; Cancer; Apoptosis
• ISSN: 1021-335X; 1791-2431
• DOI: 10.3892/or.2012.1692

Metadherin (MTDH) promotes cancer metastasis, chemoresistance, invasion and angiogenesis. Upregulation of MTDH is correlated with both progression and poor clinical outcome of many types of cancers; however, there is currently no information regarding the role of MTDH in radiation sensitivity. Here, we investigated the effects of MTDH on the radiosensitivity of cervical cancer cells using the SiHa cell line. We discovered that cervical cancer cells in which MTDH was knocked down had significantly increased radiosensitivity as measured by a clonogenic assay. MTDH knockdown cells also had increased apoptosis and a decreased proportion of cells arrested in the G2 phase after radiation treatment. MTDH knockdown also weakened the repair of DNA double-strand breaks (DSBs) induced by radiation. These results indicate that MTDH affects the radiosensitivity of cervical cancer cells and that MTDH may be a novel target to improve cervical cancer radiation response.