Development and validation of liquid chromatography–mass spectrometry method for the determination of telmisartan in human plasma

• Published in: Analytica chimica acta Vol. 540; no. 2; pp. 367 - 373
• Main Authors: Chen, Ben-mei, Liang, Yi-zeng, Wang, Ya-li, Deng, Fu-Liang, Zhou, Ping, Guo, Fang-qiu, Huang, Lan-fang
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.2005; Elsevier
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Electrospray ionization; Exact sciences and technology; LC–MS; Other chromatographic methods; Spectrometric and optical methods; Telmisartan; Electrospray ionization; LC–MS; Telmisartan; Human; Plasma; Ammonium; Ion source; Internal standard; Liquid chromatography; Acetate; Mobile phase; Electrospray; Chemical ionization; Positive ion; Vaporization; Reversed phase chromatography; LC-MS; Acetonitrile; Acetic acid; Mass spectrometry; Monitoring; Methanol
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2005.03.004

A sensitive liquid chromatographic–electrospray ionization mass spectrometric method was developed and validated for fast determination of telmisartan in human plasma. Plasma of 0.1 mL was deprotienated with methanol, centrifugation, evaporation to dryness and dissolving in mobile phase, samples were separated using a Hypersil-Keystone C18 reversed-phase column (150 mm × 2.1 mm i.d., 5 μm), together with a mobile phase containing of acetonitrile–10 mM ammonium acetate (42:58, v/v), 0.2% acetic acid and was isocratically eluted at a flow rate of 0.2 mL/min. Telmisartan and its internal standard, valsartan, were measured by electrospray ion source in positive selective ion monitoring mode. The method demonstrated linearity from 1 to 2000 ng/mL ( r = 0.9988). The limit of quantification for telmisartan in plasma was 1 ng/mL with good accuracy and precision. The mean sample extract recovery of the method were higher than 82 and 78% for telmisartan and internal standard (IS), respectively. The within-run and between-run precision ranged from 3.4 to 8.9% and 5.9 to 11.2% (relative standard deviation, R.S.D.), respectively.