Analysis of green fluorescent protein bioluminescence in vivo and in vitro using a glow discharge

The discovery of fluorescent proteins has been a revolution in cell biology and related sciences because of their many applications, mainly emphasizing their use as cellular markers. The green fluorescent protein (GFP) is one of the most used as it requires no cofactors to generate fluorescence and...

Full description

Saved in:
Bibliographic Details
Published inJournal of physics. Conference series Vol. 591; no. 1; pp. 12068 - 12071
Main Authors Hernández, L, Mandujano, L A, Cuevas, J, Reyes, P G, Osorio-González, D
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 24.03.2015
Subjects
Bioluminescence
Electron energy
Emission analysis
Emission spectra
Fluorescence
Glow discharges
Physics
Proteins
Online AccessGet full text

Cover

More Information
Summary:The discovery of fluorescent proteins has been a revolution in cell biology and related sciences because of their many applications, mainly emphasizing their use as cellular markers. The green fluorescent protein (GFP) is one of the most used as it requires no cofactors to generate fluorescence and retains this property into any organism when it is expressed by recombinant DNA techniques, which is a great advantage. In this work, we analyze the emission spectra of recombinant green fluorescent protein in vivo and in vitro exposed to a glow discharge plasma of nitrogen in order to relate electron temperature to fluorescence intensity.
ISSN:1742-6588
1742-6596
1742-6596
DOI:10.1088/1742-6596/591/1/012068